Applied Biosystems, LLC

Life Technologies Corporation

James C Nurse

Nigel P Beard

Charles Stuart Vann

Timothy G Geiser

Debjyoti Banerjee

Michael C Pallas

Theodore E Straub

Douglas Greiner

Evan Foster

Linda G Lee

Sam L Woo

Richard T Reel

David Cox

Dennis Lehto

Janusz B Wojtowicz

Jorge Fonseca

Willy K Wiyatno

Gary Lim

Mark F Oldham

Kevin Albert Maher

Eliodor G Ghenciu

Manohar R Furtado

Maxim G Brevnov

Elena Bolchakova

Gregory Govoni

Kevin Hacker

John R Van Camp

Mariela Cuadras

Johnie Young

Nikolay Sergeev

Theodore Straub

Debjyoti Dept Of Mechanical Eng Banerjee

A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. The plurality of reaction sites are each configured to include a liquid sample of at most one nanoliter. Further, the system includes a control system configured to initiate biological reactions within the liquid samples. The system further includes a detection system configured to detect biological reactions on the chip. According to various embodiments, the chip includes at least 20000 reaction sites. In other embodiments, the chip includes at least 30000 reaction sites.

Systems and methods for biological analysis

A system may include a first conduit configured to form a first batch of discrete volumes of aqueous fluid separated by spacing liquid disposed between consecutive volumes of aqueous fluid, the spacing liquid being immiscible with the aqueous fluid volumes; a second conduit, fluidically coupled to the first conduit, the second conduit configured to statically hold the first batch of discrete volumes of aqueous fluid; and a third conduit configured to receive the first batch of discrete volumes of aqueous fluid from the second conduit. The third conduit can be configured to transfer the discrete volumes of aqueous fluid of the first batch for downstream processing.

Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other

A system for processing a plurality of biological samples contains a support and a temperature controller. The suppose is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.

A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.

Electrowetting dispensing devices and related methods

A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. The sample loader further comprises a flow path between the first blade and second blade configured to dispense a liquid sample to a substrate including a plurality of reaction sites. Further, in various embodiments the liquid sample has an advancing contact angle of 85+/&#x2212;15 degrees with the first and second blade. Furthermore, loading of the liquid sample dispensed from the flow path to the plurality of reaction sites may be based on capillary action.

Systems and methods for loading liquid samples

A method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The discrete volumes are separated from one another by a liquid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

A system for processing a plurality of biological samples contains a support and a temperature controller. The support is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. The temperature controller is configured to maintain or control a temperature of at least one of the support, the case, or the one or more biological samples during an assay or reaction on the one or more biological samples. The support is also configured to maintain at least one of the surfaces of substrate at a positive angle relative to a horizontal plane during the assay or reaction.

Systems and method for biological analysis

Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.

Device for capture and lysis of microorganisms from liquids and methods of use thereof

A device having a first substrate and a second substrate separated from the first substrate to form a volume between the first and second substrates, electrodes disposed on the first and second substrates and facing the volume, each substrate comprising at least one electrode, and a first insulating layer disposed on the first substrate and a second insulating layer disposed on the second substrate to separate the electrodes from the volume.

An apparatus for controlling flow in a fluid flow path can include a chamber containing an expandable material, the expandable material being configured to expand out of the chamber into a portion of the fluid flow path so as to at least partially block the fluid flow path. The apparatus also can include at least one structure providing flow communication between the chamber and the fluid flow path. The at least one structure can be configured to pass the expandable material from the chamber to the portion of the fluid flow path during expansion so as to control a rate at which the expandable material expands into the portion of the fluid flow path.

Devices and methods for flow control in microfluidic structures

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Pleasanton, CA, United States of America

James C Nurse