USA as Represented by Secretary of the Navy

The University of Chicago

James B Delehanty

Jeffrey R Deschamps

Igor L Medintz

Philip E Dawson

Kimihiro Susumu

Eunkeu Oh

Okhil Nag

Alan L Huston

Hedi M Mattoussi

Michael H Stewart

Marc P Raphael

Jeff M Byers

Joseph A Christodoulides

Glyn Dawson

Juan Bautista Blanco-Canosa

Kelly Boeneman

Alexander L Efros

Lauren D Field

Frances S Ligler

Baochuan Lin

Brandy J White

Megan Muroski

Igor Vurgaftman

Mijin Kim

Mladen Barbic

R Joseph Weiblen

John J Macklin

Joyce A Breger

Scott Walper

Parag Chitnis

D Andrew Knight

Gregory Ellis

Kwahun Lee

Katherine Rogers

Ajmeeta Sangtani

Nashaat Rasheed

John R Cressman

Markus Muttenthaler

Bertalan K Andrasfalvy

Ryan Walters

A new seedless synthesis of anisotropic nanoscale gold nanoflower (AuNF) particles uses bidentate thiolate ligands to protect the nanoparticle surface and a combination of reagents (for example, ligand, ascorbic acid, and hydroxide) to synthesis AuNF with controlled size and anisotropic properties. Compared to prior art gold nanospheres, AuNF produced approximately a 15-fold improvement in a drug delivery assay.

Seedless synthesis of anisotropic gold nanoflowers with cellular control and drug delivery applications

Methods and systems for determining extracellular concentration data of an analyte are disclosed. A method for determining extracellular concentration data of an analyte includes receiving sensor data from one or more arrays of functionalized plasmonic nanostructures on a localized surface plasmon resonance imaging chip in contact with a fluid containing at least one living cell for a plurality of times, determining intensity data for the one or more arrays, determining fractional occupancy based on the intensity data, and determining extracellular concentration data based on the fractional occupancy data. A system for determining extracellular concentration data of an analyte includes a LSPRi chip, a sensor component, an intensity component, a fractional occupancy component, a concentration component, and a processor to implement the components.

Determining extracellular analyte concentration with nanoplasmonic sensors

A nanoparticle (NP)-peptide conjugate provides efficient steric hindrance/blockage of cellular membrane potassium (K) channels to mediate depolarization of cellular membrane potential.

Nanoparticle/peptide method of blocking potassium channels

A rhodium-loaded porphyrin complex, comprising the porphyrin (meso-tri(4-sulfonatophenyl) mono(4-carboxyphenyl)porphine (C1S3TPP)) with coordinated with rhodium, effectively neutralizes the biological activity of naturally-occurring and synthetic opioids.

Metal-porphyrin complexes for the inactivation of the biological activity of opioids

Nanoparticle-enhanced activity of a potassium channel-blocking peptide

A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be Cfullerene. Emission correlates with cellular membrane potential.

Modular, multifunctional nanoparticle-based bioconjugate for realtime visualization of cellular membrane potential

A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be Cfullerene. Photoacoustic emission from the construct correlates with cellular membrane potential.

Multifunctional nanoparticle bioconjugates for photoacoustic-based recording of cellular membrane potential

A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.

Nanoplasmonic imaging technique for the spatio-temporal mapping of single cell secretions in real time

Nanoparticles (and optionally a cargo such as a drug) can be delivered to cells by attaching just a single dendritic peptide to the nanoparticle. The dendritic peptide includes a polyhisitidine motif and a hinge and a spacer connecting the polyhistidine to a lysine-based dendritic wedge displaying at least two copies of a cell-penetrating peptide motif.

Use of single dendritic wedge cell penetrating peptides to facilitate cellular delivery of nanoparticles and nanoparticles carrying cargos

A glass pipette such as an electrode for electrophysiological recording is coated with quantum dots. This greatly aids the ability to observe the glass pipette, particular in tissue as the quantum dots provide an excellent performance under two-photon illumination used to visualize objects at depths of hundreds of microns.

Coatings of semiconductor quantum dots for improved visibility of electrodes and pipettes

Nanosplasmonic imaging technique for the spatio-temporal mapping of single cell secretions in real time

A composition having proanthocyanidin compounds having an average degree of polymerization of at least about 6. A method of administering to an immunosuppressed patient or a patient diagnosed with sepsis or septic shock a composition having a proanthocyanidin. A method of administering to a patient diagnosed with a gram negative bacterial infection a composition having proanthocyanidin compounds having an average degree of polymerization of at least about 6.

Binding interaction of proanthocyanidins with bacteria and bacterial components

A peptide attached to a nanoparticles (such as quantum dots) selectively directs the nanoparticles to neurons in a tissue or organism.

Delivery of nanoparticles to neurons

Growing community of inventors

Washington, DC, United States of America

James B Delehanty