Danisco US Inc.

Jalsen Li

Jayarama K Shetty

Bo Zhang

Sung Ho Lee

Ling Hua

Jing Ge

Zhongmei Tang

Kun Zhong

Fungal glucoamylases from&#x2014;expressed inhost cells (AfGATR) are provided.host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such asalpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed byglucoamylase (AnGA) or native AfGA expressed in. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

host cells expressing a glucoamylase fromand methods of use thereof

Growing community of inventors

Palo Alto, CA, United States of America

Jalsen Li