Carl Zeiss Microscopy Gmbh

Carl Zeiss Ag

Jakow Konradi

Daniel Schwedt

Peter Westphal

Markus Sticker

Enrico Geissler

Ingo Fahlbusch

Georg Herbst

Hans-Jürgen Dobschal

Günter Rudolph

Alexander Scheps

Timo Rojahn

A device for forming an immersion film between a front lens of a microscope objective and a preparation on an inverted microscope. The device includes a protection device for preventing fluid leakage from the immersion film region, and an apparatus for supplying the immersion fluid (auto-immersion) to the immersion film region. The protection device is arranged statically in the main part of the microscope stand and the means for supplying immersion fluid to the immersion film region is connected to the protection device by means of an Aquastop system.

Device for forming an immersion film

A method for automatic focusing of a microscope with a microscope objective on a selected area of a specimen, in which a digital hologram of the selected area of the specimen is generated in an off-axis mode and a microscope with which the method is implemented. The digital hologram is used to determine, on the optical axis of the microscope objective, a focus position to be set in which the selected area of the specimen is optimally in focus. Subsequently, a control system is used to set the microscope to the focus position determined and thus is focused on the area selected.

Method and device for focussing a microscope automatically

Laser scanning microscope or spectral detector having a detection beam path and first imaging optics which image spectrally dispersed sample light in a Fourier plane such that the individual spectral components of the sample light are spatially separated from one another therein. A micromirror arrangement is provided in this plane, and a spectrally selective change in direction of the detection beam is carried out by controlling the micromirrors, where a useful light component of the detection beam arrives on a detector. At least one second micromirror arrangement and a 1:1 imaging of the first micromirror arrangement in the second micromirror arrangement is provided. Alternatively, the same micromirror arrangement is passed at least twice, where, in the light path between the first pass and second pass, a spatial offset of the light beam of at least the first pass and second pass is generated on the micromirror arrangement by optical means.

Spectral detector or laser scanning microscope having variable filtration using spatial color separation

An optical filter device having a polarizing beamsplitter, an achromatic polarization manipulator, and at least one dichroic mirror. The polarization manipulator is arranged optically between a first input/output of the polarizing beamsplitter and the dichroic mirror. The polarization manipulator is also constructed in such a way that it effects a 90-degree rotation of a polarization direction of light which a) exits the polarizing beamsplitter at the first input/output, b) traverses the polarization manipulator, c) is reflected by the dichroic mirror, and d) again traverses the polarization manipulator. The optical filter device can also be used as beamsplitter.

Optical filter device, in particular for microscopes

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Erfurt, Germany

Jakow Konradi