Buhlmann Laboratories Ag

Jakob Weber

Thomas Jermann

Daniel Gygax

Lara Hasan

Michel-Angelo Sciotti

Erik Pavels Petersen

André Scholer

Gregor Naef

Marie-Eve Überschlag

Marianne Prica

Arne Roseth

Jean Sainte-Laudy

Michael Schneider

Lukas Steiner

Cédric Wernli

Frank Bantleon

Andreas Abel

A hand-held applicator () for collecting, mixing, diluting and discharging a sample liquid, comprising a main body () having an inlet-aperture (), an outlet-aperture () and a chamber () for storing a liquid solution, wherein the chamber is connected to the inlet-aperture and the outlet-aperture, a lid () for covering the inlet-aperture and a tube (), moveably supported in the inlet-aperture when the lid is covering the upper portion of the main body, wherein the tube () is moveable between a predetermined position outside the chamber and a position, in which the tube is at least partly located in the chamber.

Hand-held applicator

The present invention concerns a method for determining the concentration of a protein in a gastrointestinal (GI) tract sample taken from a human or an animal. The present invention is characterized by the feature that a dilution of the sample in the buffered aqueous extraction medium in a range of 1:100 to 1:10,000 is obtained. The present invention leads to a significant improvement of the technical situation, and provides a simple, sensitive and specific determination tool of proteins in GI tract samples. The determination of proteins, e.g. calprotectin, elastases or hemoglobin, in GI tract samples leads to more accurate and reproducible results.

Method for determination of a protein

A tube for extracting, diluting and discharging a sample: able to control the amount of discharged diluted solution in the tube, after the sample has been diluted; that improves the hygienic conditions of discharging the sample; that enables introduction of a predetermined amount of a sample in any liquid or solid state; and that is able to transfer a specific amount of a diluted sample into another tube for further processing without any hygienic problems and with no risk of accidents.

Sample extracting, diluting and discharging device

The invention relates to methods for the determination of vitamin B6 in samples as well as to reagent compositions for assaying a sample for vitamin B6 and to a test kit suitable for carrying out the methods according to the present invention. Further, the invention relates to the use of such methods for the application to different analyzing devices such as micro-titer plate readers and fully automated clinical chemistry analyzers (autoanalyzers).

Method for the determination of the concentration of vitamin B6 in a sample

Methods to determine the concentration of gamma-hydroxy butyric acid (GHB) in a sample as well as compositions and a kit suitable for carrying out said methods. Also, the use of the methods for application on a microtiter plate or an auto-analyzer.

Methods for determining the concentration of gamma-hydroxy butyric acid (GHB) in a sample

The invention pertains to a method for the determination of basophil activation induced by a test substance by flow cytometric measurement of the changes of the mean or median fluorescence intensities (MFI) of the basophilic F&#x3b5;RI receptor present on the cell surface of basophils (MFI-F&#x3b5;RI) and/or the IgE antibodies bound to the F&#x3b5;RI receptor (MFI-IgE), and the CD63 antigen exposed on the cell surface of basophils after their activation (MFI-CD63), by means of a mixture of anti-CD63, anti-F&#x3b5;RI or anti-IgE, and anti-CCR3 antibodies each labelled with a distinct fluorophore, of which at least one antibody acts as a basophil selection marker and at least two antibodies act as basophil activation markers, and bringing the mean fluorescence intensities of the activation markers in correlation to obtain an Activation Index. These methods combining the measurement of an early (such as IgE, F&#x3b5;RI or CD203c) and a late basophil activation marker (such as CD63), respectively, provide a markedly improved clinical sensitivity in allergy diagnosis over existing methods which consider only one activation marker, such as CD203c or CD63, expressed in percentage of basophil activation. It is also an aspect of the present invention to provide an ex-vivo allergy provocation test comprising the above-mentioned flow cytometric measurement and analysis of the results as well as a test kit for carrying out the test in-vitro.

Allergy test based on flow cytometric analysis

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Reinach, Switzerland

Jakob Weber