Life Technologies Corporation

Applied Biosystems, LLC

Jaiprakash G Shewale

Manohar R Furtado

Maxim G Brevnov

Hemant S Pawar

Pius Brzoska

Jason Yingjie Liu

Rixun N Fang

James L Ratcliff

James Stray

Michael Malicdem

William E Cooley

Allison Holt

Maura Barbisin

Cristin O'Shea

Esmeralda Ann Garcia-Smith

Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. The nucleic acids can be separated by forming a stable complex with soluble polysaccharide polymers and magnetic particles, in the presence of detergents and solvent. When the particles are magnetically separated out of the solution, the nucleic acids are separated with them. The nucleic acids can subsequently be released and separated from the particles. The nucleic acid preparation is useful for achieving efficient and accurate results in downstream molecular techniques such as quantification, identification of the source of the nucleic acids, and genotyping.

Methods and kits for extraction of DNA

The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

Lysis buffers for extracting nucleic acids

The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.

The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.

Multiplex compositions and methods for quantification of human nuclear DNA and human male DNA and detection of PCR inhibitors

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Santa Clara, CA, United States of America

Jaiprakash G Shewale