Sigma-aldrich Co. LLC

Jaime K Robert

Carol A Kreader

Brian W Ward

Kenneth E Heuermann

Kenneth B Heuermann

Methods for primer switching during amplification reactions are provided. In particular, methods are provided for converting single primer PCR amplicons to dual primer PCR amplicons.

Single primer to dual primer amplicon switching

Processes, oligonucleotides, and kits for amplifying RNA. In particular, the processes generate and amplify cDNA libraries in which the orientation of the input RNA molecule is preserved in the products. Among the various aspects of the present disclosure is the provision of process for directionally amplifying RNA. The process comprises reverse transcribing at least one RNA molecule in the presence of a plurality of first synthesis primers to generate a plurality of first strands of complementary DNA (cDNA), wherein each of the first synthesis primers comprises a 3' sequence having complementarity to a portion of the RNA molecule, a non-complementary 5&#x2032; sequence corresponding to one or more amplification primers, and optionally an internal tag sequence comprising a first tag sequence.

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St. Louis, MO, United States of America

Jaime K Robert