University of Pennsylvania

Agilent Technologies, Inc.

Jai-Yoon Sul

James H Eberwine

Junhyong Kim

Philip G Haydon

Chia-Wen Wu

Fanyi Zeng

Ivan Julian Dmochowski

Ulo Langel

Julianne C Griepenburg

Teresa L Rapp

Vickas Patel

Hajime Takano

Tae Kyung Kim

Emelia Eiriksdottir

Jaehee Lee

Jennifer M Singh

Sean B Yeldell

Kevin Miyashiro

Jinchun Wang

Mimi Healy

Tiina Peritz (nee Pajunen)

Youtao Lu

Jifen Li

Stephen Fisher

Jae-Hee Lee

Thomas J Bell

Tina Peritz

Kitty Chia-Wen Wu

Chia-Wen Kitty Wu

Compositions and methods of capturing one or more nucleic acid molecules of a cell or subcellular compartment are described. In certain aspects, the compositions comprise a caged molecule comprising one or more photolinkers and an antisense oligonucleotide, which when uncaged hybridizes to a target nucleic acid molecule.

Transcriptome in vivo analysis (TIVA) and transcriptome in situ analysis (TISA)

Provided herein are compositions and methods to assess the genomic landscape of fixed cells using light activated oligonucleotides that can be directed to the nucleus, mitochondria, or cytoplasm of fixed cells and that, upon activation, can be extended for in situ copying of nuclear single-stranded DNA (i.e., open chromatin), open mitochondrial DNA, and/or cytoplasmic RNA into barcoded complementary DNA. These methods also provide for gene specific 3D chromatin structural niche analysis.

Compositions and methods for genomic DNA and gene expression analysis in single cells

The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.

Transcriptome transfer produces cellular phenotype conversion

The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the transfected cell.

Methods for phototransfecting nucleic acids into live cells

The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.

Methods for transfecting nucleic acid into live cells

The present invention includes compositions, methods and kits for the real-time detection of transcription and translation in live cells, tissues and organisms. The present invention further provides method for the rapid sequencing of nucleic acids without using conventional sequencing techniques or reactions.

Compositions, methods and kits for real-time nucleic acid analysis in live cells

Methods of detecting transcription

The invention relates to a method of modulating neuronal function by modulating the cytoplasmic level in a neuron of an intron-containing mRNA. The methods are useful in diagnostic, research and therapeutic applications.

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Bensalem, PA, United States of America

Jai-Yoon Sul