The United States of America as Represented by the Secretary of

Jagdev M Sharma

Tommy L Fredericksen

Ben R Burmester

A substantially pure species of avian Interleukin-2 has a molecular weight of about 30 kda. as determined by SDS-polyacrylamide gel electrophoresis. The compound is obtained from avian lymphocytes. It is produced by collecting lymphocytes from an avian donor, growing the lymphocytes in a medium containing a T cell mitogenic agent, and recovering the compound from the medium.

Avian interleukin-2

Avian diseases, particularly those which threaten birds early in life, are controlled by embryonal vaccination during the final quarter of incubation. The site of inoculation may be either into the amnion, to include the amniotic fluid and the embryo itself, or else into the yolk sac. Protection afforded to chicks inoculated prehatch is four times or more than that of chicks inoculated post-hatch.

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Okemos, MI, United States of America

Jagdev M Sharma