Korea Telecommunication Authority

Korea Basic Science Institute

Jae Yong Lee

Jong Shin Yoo

Jin Young Kim

Yeong Hee Ahn

Kun Cho

Kyoung Kuen Lee

Yi Han Kim

Byung Chul Kim

Young Do Joo

Kun 0Ho

The present invention relates to a method for phosphorylation site-specific labeling of phosphoproteome with a site-specific tagging reagent and analyzing of the resulting labeled one, more especially, a method for in-situ tagging of phosphorylation sites of phosphoproteins retained in polymeric gel with a nucleophilic tagging reagent. It also relates a method for generating new proteolytic cleavable sites at formerly phosphorylation sites by a proper choice of a nucleophilic tagging reagent. It also relates to a method for phosphopeptides analysis and phosphorylation site identification by in-gel digestion of the previously in-gel tagged proteins and subsequent mass analysis of the resulting peptides. The invention provides in-gel chemical tagging method for phosphoaminoacid residue of phosphoproteins retained in polymeric gel matrix. Phosphoprotein can be immobilized into gel matrix by a variety of methods such as gel electrophoresis. The immobilized phosphoproteins are retained in gel matrix during tagging reaction to phosphorylated aminoacid residue of phosphoproteins, and the resulting tagged proteins are also retained in gel matrix till following purification steps like washing of the tagging reagents are accomplished. The tagged proteins is digested by protease, and the resulting digested peptides is released from gel into solution and applied for peptide mass analysis. 

In-gel tagging and in-gel digestion for phosphoproteins analysis and phosphorylation site identification

Disclosed is a method for high speed rerouting in a multi protocol label switching (MPLS) network which can minimize a packet loss and enable a fast rerouting of traffic so as to protect and recover a multi point to point LSP occupying most LSPs in the MPLS network. The method for high speed rerouting in a multi protocol label switching (MPLS) network, the method comprising the steps of controlling a traffic stream to flow in a reverse direction in a point where a node or link failure occurs by using a backup Label Switched Path (LSP) comprising an Explicitly Routed (ER) LSP having a reverse tree of a protected multi point to point LSP and an ingress LSR through an egress LSR.

Method for high speed rerouting in multi protocol label switching network

Growing community of inventors

Daejeon-Shi, South Korea

Jae Yong Lee