Centre National De La Recherche Scientifique

Commissariat a L'energie Atomique

Genoplante-valor

Jacques Joyard

Eric Marechal

Stéphane Miras

Daniel Salvi

Norbert Rolland

Myriam Ferro

Didier Grunwald

Jerome Garin

Roland Douce

Jérome Garin

Maryse Block

The invention relates to a non-cleavable, plastidial targeting polypeptide derived from a protein from the inner membrane of plant chloroplasts. Said peptide is particularly suitable for importing proteins of interest in plants.

Plastidial targeting peptide

The invention concerns membrane fractions of cells containing a recombinant MGDG synthase and enriched with 1,2-sn-diacylglycerol, their preparation method, their use for screening molecules inducing MGDG synthase activity and a method for screening molecules inducing MGDG synthase activity using said membrane fractions.

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The invention relates to a non-cleavable, plastidial targeting polypeptide derived from a protein from the inner membrane of plant chloroplasts. Said peptide is particularly suitable for importing proteins of interest in plasts.

Plastidial-targeting peptide

The invention concerns a method for screening and selecting parasiticides (apicomplex phyllum parasites) and/or herbicides and the uses thereof. Said method consists in incubating a substance to be tested with a MGDG synthase and measuring the specific enzymatic activity, after said incubation. The invention also concerns the use of MGDG synthase or a plant isolated plastid membrane for selecting or screening the products inhibiting the activity of the MGDG synthase, capable of being used as active principles against apicomplex parasites and/or of being used as herbicides.

Screening method involving MGDG synthase

Growing community of inventors

Meylan, France

Jacques Joyard