Commissariat a L'energie Atomique

Jacques Grassi

Jean-Philippe Deslys

Emmanuel Comoy

Philippe Pradelles

The invention relates to a method for diagnosing a transmissible spongiform subacute encephalopathy (TSSE) caused by an unconventional transmissible agent (UTA) or prion. The method involves treating a sample suspected of containing a prion with proteinase K for a time and under conditions that completely degrade normal prion protein (Prp-sen), but which only partially digest abnormal prion protein (PrP-res) so that all or some of the octapeptide motif repeats comprising P(H/Q)GGG(-/T)WGQ (SEQ ID NO: 1) in the abnormal prion protein (Prp-res) are retained.

Method for diagnosing a transmissible spongiform subacute encephalopathy caused by an unconventional transmissible agent strain in a biological sample

Method for diagnosing a transmissible spongiform subacute encephalyopathy caused by an unconventional transmissible agent strain in a biological sample

Compound labelled by the acetyl cholinesterase of Electrophorus electricus, its preparation process and its use in enzymoimmunology. This compound is constituted by a molecule chosen from among the antigens, haptens and antibodies, bonded by a covalent bond to an enzyme formed by the acetyl cholinesterase of Electrophorus electricus (electric eel). For example, the molecule is the substance P or a prostaglandin and the compound can be used for the enzymoimmunological determination of these molecules.

Growing community of inventors

Bures-sur-Yvette, France

Jacques Grassi