Nestec S.a.

Jacques Edouard Germond

Beat Mollet

Luciane LaPierre

Raymond David Pridmore

Blaise Corthesy

Herbert Hottinger

Michele Delley

Rodolphe Fritsche

Olivier Mignot

Olivier Marciset

Koichiro Tsuda

Mich&egrave;le Delley

The present invention pertains to novel strains of lactic acid bacteria that are capable to reduce an individual's tendency to mount allergic reactions. In particular, the present invention relates to the generation of lactic acid bacteria strains that express proteins including tolerogenic peptides and the use thereof in reducing an individual's tendency to mount allergic reactions. The invention also pertains to food or pharmaceutical compositions containing said micro-organisms or active fractions thereof.

Lactic acid bacteria capable of reducing an individual's tendency to develop allergic reactions

A DNA sequence suitable for the controlled transcription and/or expression of a variety of different genes in bacteria, preferably gram positive bacteria is provided. In particular, the DNA sequence includes the promoter and the gene coding for the lac repressor of the lac operon ofwith a DNA sequence coding for a gene product of interest being arranged inbetween.

Expression contructs using Lactobacillus delbrueckii subsp. lactis lac repressor protein and its lac repressor binding site, microorganisms and methods thereof

The use of a IS-element as a tools for genetically modifying the genome of,or,. An IS-element, originating from,selected from the DNA sequences SEQ ID NO: 7-10 or functional derivatives thereof. A,or,providing a gene encoding, or affecting the production of, enzymes producing secondary metabolites, the &bgr;-galactosidase, the cell wall protease, the catabolite control protein A, the lactate dehydrogenase, a glycosyltransferase, a restriction system, a lysogenic prophage, or the permease of the lac operon, wherein the gene is inactivated by insertion of at least one IS-element. The use of the above-mentioned,or,for the preparation of a fermented product.

Mobile genetic elements as tools for genetic modification of L. delbrueckii or L. helveticus

Recombinant strains of,which have capacities of surviving passage through the intestine, adhering to human intestinal cells and increasing phagocytosis of macrophages and which have a sequence of gene coding which is an,strain sequence of gene coding for D-lactate dehydrogenase modified so that the recombinant strain produces only L(&plus;)-lactate.

Recombinant, sequence-modified L. johnsonii bacteria which produce only L(&plus;)-lactate

A recombinant strain of L. johnsonii which has capacities of surviving passage through the intestine, adhering to human intestinal cells and increasing phagocytosis of macrophages and which has a gene sequence code for D-lactate dehydrogenase which is a modified L. johnsonii gene sequence code and which produces only L(+)-lactate.

Recombinant sequence-modified L. johnsonii bacteria which produce only   +

Bacteriocins from Streptococcus thermophilus

The present invention concerns Lactobacillus bulgaricus wherein the lactate dehydrogenase activity is lower than 50%, preferably lower than 10%, of the lactate dehydrogenase activity of Lactobacillus bulgaricus wild type strain. The present invention concerns also the food composition comprising said Lactobacillus bulgaricus.

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Crissier, Switzerland

Jacques Edouard Germond