Diatron Corporation

Diatron Diagnostics Corporation

Jacques Claude Levin

Walter B Dandliker

June K Dandliker

Light, pulsed or continuous at a wavelength (e.g. 780 nm), fluoresceces a specimen. The specimens may be combinations of a dye (preferably labelled), an antigen (e.g. rubella) and an antibody reactive with the antigen, with properties of polarizing the light when fluoresced. The light polarized in a first direction (e.g. z-axis) parallel to the incident light and in a second direction (e.g. x-axis) perpendicular to the incident light are measured. A second specimen is then provided with the antigen and the antibody but without the dye. The same light as discussed above fluoresces the second specimen and polarizes the light when fluoresced. The light polarized in the first (z-axis) and second (x-axis) directions in the second specimen is measured. These measurements are processed in a microprocessor with the measurements in the z and x directions in the first specimen to identify the antigen or, when the antigen is known, to identify the concentration of the antigen in the first specimen. When the light is pulsed, the measurements are made in a time window beginning after the initiation, and terminating before the end, of the fluorescence of the combination of the dye, the antibody and the antigen. When the light is continuous, it is modulated. Measurements are made of the phase shifts in the polarized light in the z and x directions as a result of the light modulations.

Transient state luminescene assay apparatus

Light, pulsed or continuous at a particular wavelength (e.g. 780 nm), fluoresces a specimen. The specimen may be combinations of an antigen (e.g. rubella) labelled with a fluorescent dye, unlabeled antigen or hapten and an antibody reactive with the antigen or hapten. The light polarized in a first direction (e.g. z-axis) parallel to the electric field of the incident light and in a second direction (e.g. x-axis) perpendicular to the first direction is measured. A second specimen is then provided with the antigen and the antibody but without the dye. The same light as discussed above excites the second specimen and polarizes the light. The light polarized in the first (z-axis) and second (x-axis) directions in the second specimen is measured. These measurements are processed in a microprocessor with the measurements in the z and x directions in the first specimen to identify the antigen or, when the antigen is known, to identify the concentration of the antigen in the first specimen. When the light is pulsed, the measurements are made in a time window beginning after the initiation, and terminating before the end, of the fluorescence of the combination of the dye, the antibody and the antigen. Determinations as discussed above but without unlabeled antigen or hapten may be made of the antibody instead of the antigen. When the light is continuous, it is modulated. Measurements are made of the phase shifts in the polarized light in the z and x directions as a result of the light modulations and the decay of the fluorescence.

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Fort Lauderdale, FL, United States of America

Jacques Claude Levin