Promega Corporation

Jacqueline Sankbeil

Keith V Wood

Erika Hawkins

Rex M Bitner

Braeden L Butler

John M Centanni

Craig E Smith

Douglas H White

The invention provides kits and methods for increasing the sensitivity of a bio-luminescent assay, which employ an organic compound that, for instance, reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold and reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold, reduces luminescence generated by luminogenic molecules not bound to an enzyme by at least about 10 fold and reduces the luminescence generated by luminogenic molecules bound to an enzyme by less than about 7 fold, or reduces autoluminescence by at least about 10 fold and reduces luminescence that is dependent on the presence of an analyte by less than about 7 fold.

Method for increasing luminescence assay sensitivity

A method for increasing the sensitivity of a bio-luminescent assay comprising carrying out the assay in the presence of an organic compound that reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold, and that reduces, maintains, or increases the luminescence that is dependent on the presence of an analyte.

Assay kits for increasing the sensitivity of luminescent assays are provided that include an organic compound that reduces luminescence that is not dependent on the presence of an analyte by at least about 10 fold, and that reduces, maintains, or increases the luminescence that is dependent on the presence of an analyte.

Growing community of inventors

Edgerton, WI, United States of America

Jacqueline Sankbeil