Angiomed Gmbh & Co. Medizintechnik Kg

Angiomed Gmbh & Co. Medizentechnik Kg

Jacobus Henricus Veerkamp

Thiemo Blank

Antonius H M S M Van Kuppevelt

Antonius Henricus Minardus Severus Marie Van Kuppevelt

Christiaan Hendrikus Adriaan Van De Lest

The invention relates to a method for linking nucleic acid and/or glycosaminoglycan or glycosaminoglycan mimetics to a polar/hydrophilic material, characterized by contacting a nucleic acid and/or glycosaminoglycan and a polar/hydrophilic material with each other in the presence of a solution being 20 to 100 percent saturated with a non-chaotropic salt and removing said solution from the nucleic acid and/or glycosaminoglycan&#x2014;polar/hydrophilic material.

Method for linking nucleic acids and/or glycosaminoglycans to polar/hydrophilic materials

A method for linking strongly negatively charged biological macromolecules like deoxyribonucleic acid (DNA), ribonucleic acid (RNA) and glycosaminoglycans (GAG) to plastic substrates, comprising contacting the macromolecules and the plastics with a non chaotropic solution containing a salt in an amount of at least 20% of its saturation concentration, or having a pH below the pKa of the charged groups of the macromolecules to be linked. Also disclosed are the products of the method, in particular a microtiter plate with plastic wells coated with the negatively charged non-proteinaceous macrobiomolecules.

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Nijmegen, Netherlands

Jacobus Henricus Veerkamp