Pioneer Hi-Bred International, Inc.

E.i. Dupont De Nemours and Company

Other

University of California

Curagen Corporation

Jacob T Gilliam

Joyce R Maddox

Jonathan P Duvick

Otto Folkerts

Oswald R Crasta

Nasser Yalpani

Jon Duvick

Eric J Schepers

Daniel J Altier

Carl R Simmons

James Kevin Presnail

Tamas Torok

Jennie Hunter-Cevera

Hua Dong

Jennifer Kara Barry

James J English

Thomas Chad Wolfe

Natalia Ellanskaya, Legal Representative

Irina Ellanskaya

Kai M Hillman

Daniel James Thorpe

Benjamin A Bowen

Xun Wang

Aragula Gururaj Rao

Tracy A Rood

Virginia C Crane

Edmund H Crane, Iii

I A Ellanskaya

Petr Karlovsky

IA Ellanskaya

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Insecticidal proteins and methods for their use

Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for family members of a novel family of antipathogenic polypeptides. Polynucleotides that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the polynucleotides disclosed herein is further provided. Compositions comprising an antipathogenic polypeptide or a microorganism comprising an antipathogenic polynucleotide of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Plants, plant cells, seeds, and microorganisms comprising an antipathogenic polnucleotide or polypeptide of the invention are also disclosed.

Antipathogenic proteins and methods of use

Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include an amino acid sequence, and variants and fragments thereof, for an antipathogenic polypeptide that was isolated from a fungal fermentation broth. Nucleic acid molecules that encode the antipathogenic polypeptides of the invention, and antipathogenic domains thereof, are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

Nucleic acids encoding antifungal polypeptides and uses thereof

Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

Isolated nucleic acids encoding antipathogenic polypeptides and uses thereof

The invention relates to compositions including amino acid sequences isolated from fungal fermentation broths and methods for protecting a plant from a pathogen, particularly a fungal pathogen.

Antifungal polypeptides

The invention relates to antifungal compositions and methods for protecting a plant from a fungal pathogen. Compositions including antifungal polypeptides isolated from a fungal fermentation broth are provided.

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated fromand. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use

The present invention provides transformed plants comprising a polynucleotide enconding amino polyol amine oxidase (APAO ) fromand a method of expressing APAO transgenic plants and seeds. In this way, transgenic plant can be produced having fumonisin degrading capability, as well as with the capability of producing the degrading enzymes.

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from . Additionally, the polynucleotide encoding for the APAO enzyme can be used to transform plant cells normally susceptible to or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems.

Amino polyol oxidase amine polynucleotides and related polypeptides and methods of use

Compositions and methods for the complete detoxification of fumonisin and fumonisin degradation products are provided. Particularly, nucleotide sequences corresponding to the detoxification enzymes are provided. The sequences find use in preparing expression cassettes for the transformation of a broad variety of host cells and organisms.

Compositions and methods for fumonisin detoxification

The present invention provides nucleotide sequences encoding zearalenone detoxification polypeptides, and methods for mycotoxin detoxification using the sequences. One method comprises stably incorporating into the genome of a plant cell, a nucleotide sequence of the present invention operably linked to a heterologous promoter and regenerating a stably transformed plant that expresses the nucleotide sequence.

Compositions and methods of zearalenone detoxification

The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from and . Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium. Plants can be regenerated from the transformed plant cells.

Growing community of inventors

Norwalk, IA, United States of America

Jacob T Gilliam