Monsanto Company

Monsanto Technology LLC

Solutia Incorporated

Pharmacia Corporation

Monsanto Technologies LLC

Jacob S Tou

Alfred A Schleppnik

Jessica Marie Ballinger

S Bradley Storrs

Douglas W Taylor

Dennis J Kalota

Yueting Chou

Billy D Vineyard

David C Silverman

Timothy K Hirzel

Winsor R Cho

This invention describes a method of removing N-terminal alanine residues from polypeptides, preferably recombinant proteins, using an aminopeptidase derived from the marine bacterium . Accordingly, Aeromonas aminopeptidase (AAP; E.C. 3.4.11.10) can be used to remove N-terminal alanyl residues from derivatives of human somatotropin (hST, human growth hormone, or hGH), porcine somatotropin (pST), and bovine somatotropin (bST), for example, to yield proteins having their native amino acid sequences. The enzyme reactions can be carried out in free solution, or the AAP can be immobilized on a solid support, for reactions carried out in vitro. An efficient method for converting Ala-hGH to hGH, for example, comprises expression of Ala-hGH in , recovery of inclusion bodies, solubilization and refolding in detergent, detergent removal by ultrafiltration, selective precipitation, enzyme cleavage, followed by two column chromatography steps.

Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase

This invention describes a method of removing N-terminal alanine residues from polypeptides, preferably recombinant proteins, using an aminopeptidase derived from the marine bacterium . Accordingly, (AAP; E.C. 3.4.11.10) can be used to remove N-terminal alanyl residues from derivatives of human somatotropin (hST, human growth hormone, or hGH), porcine somatotropin (pST), and bovine somtotropin (bST), for example, to yield proteins having their native amino acid sequences. The enzyme reactions can be carried out in free solution, or the AAP can be immobilized on a solid support, for reactions carried out in vitro. An efficient method for converting Ala-hGH to hGH, for example, comprises expression of Ala-hGH in , recovery of inclusion bodies, solubilization and refolding in detergent, detergent removal by ultrafiltration, selective precipitation, enzyme cleavage, followed by two column chromatography steps.

Method of removing N-terminal alanine residues from polypeptides with Aeromonas aminopeptidase

This invention relates to novel water soluble metal working fluid compositions, their use to work metal, a process for working metal using such compositions and the metal worked article of manufacture. More particularly, this invention relates to fluid compositions useful in cutting, grinding, shaping and other metal working operations which require a lubricant. The terms &ldquo;first Group A&rdquo; and &ldquo;second Group B&rdquo; are used herein to denote different groups and not to indicate any sequence of use or selection as any possible combination or sequence of use of a component(s) is envisioned without limit of any kind. The disclosed fluid compositions are also anticorrosive and environmentally more acceptable than current oil based fluids. There has now been discovered an essentially odorless, substantially non-oil misting, water-soluble metal working fluid comprising at least one component selected from a first Group A herein and optionally one or more components selected from a second Group B herein preferably with the balance of the composition being water and other (optional) minor ingredients. When a component is employed from Group A and a component is employed from Group B the action of the combination generally enhances performance of the resulting combination with contain moieties from both Group A and Group B.

Water soluble metal working fluids

A method for the solubilization and/or naturation of a somatotropin involves contacting a somatotropin with a detergent composition and water under conditions effective to obtain a naturated somatotropin, wherein the detergent composition may be a C , C , C or C acyl glutamate, a C , C or C alkyl sulfate, an alcohol ethoxy sulfate, lauroyl ethylenediamine-triacetic acid (LEDA), a C to C linear alkyl benzene sulfonate, diphenyl disulfonate or an acyl amino acid.

Method of solubilization and naturation of somatotropins

Method for solubilization and naturation of somatotropins

A method for the solubilization and/or naturation of a somatotropin involves contacting a somatotropin with a detergent composition and water under conditions effective to obtain a naturated somatotropin, wherein the detergent composition may be a C.sub.10, C.sub.12, C.sub.16 or C.sub.18 acyl glutamate, a C.sub.10, C.sub.14 or C.sub.18 alkyl sulfate, an alcohol ethoxy sulfate, lauroyl ethylenediamine-triacetic acid (LEDA), a C.sub.10 to C.sub.18 linear alkyl benzene sulfonate, diphenyl disulfonate or an acyl amino acid.

A regioselective process for the preparation of .alpha.-L-aspartyl-L-phenylalanine methyl ester is disclosed. A controlled aqueous coupling reaction between .beta.-methyl-L-aspartate-N-carboxyanhydride and L-phenylalanine produces the aspartyl methyl ester of .alpha.-L-aspartyl-L-phenylalanine which is subsequently hydrolyzed and selectively esterified without isolation. The hydrochloride salt of .alpha.-L-aspartyl-L-phenylalanine methyl ester, which is selectively precipitated from the esterification mixture, can be neutralized to .alpha.-L-aspartyl-L-phenylalanine methyl ester.

Process for the preparation of .alpha.-L-aspartyl-L-phenylalanine methyl

A phenylethyl substituted dimer of ethylene and maleic anhydride, named 7-phenyloctane-(1,2),(5,6)-dianhydride, is chemically synthesized by

Cyano-dicarboxylate

Cyano-polycarboxylates

A phenylethyl substituted dimer of ethylene and maleic anhydride, names 7-phenyloctane-(1,2),(5,6)-dianhydride, is chemically synthesized by

Chemical synthesis of ethylene/maleic anhydride dimer with phenylethyl

Mono-substituted terminal diesters of malonic acid are prepared by reaction of the corresponding substituted 1,2-diesters with alkali metal hydride in the presence of organic solvent medium.

Growing community of inventors

Ballwin, MO, United States of America

Jacob S Tou