Flagship Pioneering Innovations Vi, LLC

Flagship Pioneering Innovations V, Inc.

Children's Medical Center Corporation

Dsm Nutritional Products Inc.

Kaleido Biosciences, Inc.

Flagship Pioneering Innovations Vii, LLC

Beth Israel Deaconess Medical Center, Inc.

Sana Biotechnology, Inc.

Jacob Rosenblum Rubens

Geoffrey Von Maltzahn

John M Geremia

Pedro J Del Nido

John Miles Milwid

Barrett Ethan Steinberg

Robert James Citorik

William Edward Salomon

Zi Jun Wang

Michael Travis Mee

Jared Silverman

Yvonne J Yamanaka

Kiana Mahdaviani

Kyle Marvin Trudeau

Anne Helen Bothmer

Randi Michelle Kotlar

James D McCully

Robert Charles Altshuler

Ananya Ray

Nathaniel Roquet

Cecilia Giovanna Silvia Cotta-Ramusino

Douglas B Cowan

Neal Gordon

Molly Krisann Gibson

Kyusik Kim

Jacob Feala

Stephen Hoyt Cleaver

David Chess

Donghui Li

Sandeep Kumar

Bo Zhang

Nathan Wilson Stebbins

Sidney Levitsky

Brigham Jay Hartley

Benjamin Andrew Portney

Narahari Subbanna Pujar

Tamar Rose Putiri

Alexandra Rachael Sneider

Jagesh Vijaykumar Shah

Matthew Milnes Dobbin

Camilo Ayala Breton

Jack Milwid

Sitaram M Emani

Neal Francis Gordon

Geoffrey A Von Maltzahn

DouglasB Cowan

Michael Mee

Methods and compositions for modulating a target genome are disclosed. This disclosure relates to novel compositions, systems and methods for altering a genome at one or more locations in a host cell, tissue or subject, in vivo or in vitro. In particular, the invention features compositions, systems and methods for inserting, altering, or deleting sequences of interest in a host genome.

Methods and compositions for modulating a genome

Methods and compositions for modulating a target genome are disclosed. The composition may comprise a first RNA encoding a polypeptide comprising a retrotransposase reverse transcriptase domain and a retrotransposase endonuclease domain. The composition may also comprise a second RNA comprising a sequence that binds the polypeptide and a heterologous object sequence. The composition may insert the sequence of the heterologous object sequence into a target DNA.

The present disclosure provides, at least in part, methods and compositions for in vivo fusosome delivery. In some embodiments, the fusosome comprises a combination of elements that promote specificity for target cells, e.g., one or more of a fusogen, a positive target cell-specific regulatory element, and a non-target cell-specific regulatory element. In some embodiments, the fusosome comprises one or more modifications that decrease an immune response against the fusosome.

Fusosome compositions and uses thereof

Preparations of glycan therapeutics, pharmaceutical compositions, dietary supplements and medical foods thereof are provided, and methods of using said gycan therapeutics, e.g. in cancer therapy.

Glycan therapeutic compositions and related methods thereof

The disclosure provides, for example, single stranded, covalently closed DNA that does not form a double stranded structure longer than 100 base pairs. The ssDNA may encode an effector sequence, for instance a therapeutic protein. The ssDNA may comprise a nuclear targeting sequence (NTS). In some embodiments, the ssDNA shows decreased activation of the innate immune system compared to an otherwise similar dsDNA.

DNA compositions and related methods

Methods and compositions for modulating a target genome are disclosed.

Therapeutic chondrisome and mitoplast compositions and related methods are described.

Methods and compositions relating to chondrisomes from blood products

Therapeutic chondrisome compositions and related methods are described.

Methods and compositions relating to chondrisomes from cultured cells

Preparations of glycan therapeutics, pharmaceutical compositions, dietary supplements and medical foods thereof are provided, and methods of using said glycan therapeutics, e.g. in cancer therapy.

In some aspects, fusosome compositions and methods are described herein that comprise membrane enclosed preparations, comprising a fusogen. In some embodiments, the fusosome can the target cell, thereby delivering complex biologic agents to the target cell cytoplasm.

Compositions for facilitating membrane fusion and uses thereof

Growing community of inventors

Cambridge, MA, United States of America

Jacob Rosenblum Rubens