Waters Technologies Corporation

Brousmiche, Darryl W.

Wyndham, Kevin Daniel

Hill, Jason F.

Fairchild, Jacob Nathan

Jacob Nathan Fairchild

Kevin Daniel Wyndham

Jason F Hill

Michael F Morris

Giorgis Isaac

Joseph D Michienzi

Matthew A Lauber

Darryl W Brousmiche

Wade P Leveille

Jeffrey Musacchio

Michael O Fogwill

Geoff C Gerhardt

Theodore A Dourdeville

Pamela Carmen Iraneta

Nicole L Lawrence

Stephen J Shiner

Daniel P Walsh

Curt Devlin

Bonnie A Alden

Christopher J Hudalla

Dimple D Shah

Dimple Shah

Euan Ross

Paul Rainville

Benjamin Wuyts

Babajide Okandeji

The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group. In some examples, the present disclosure can include a chromatographic system having a chromatographic column having a stationary phase with a chromatographic substrate containing silica, metal oxide, an inorganic-organic hybrid material, a group of block copolymers, or a combination thereof.

Chromatographic materials for the separation of unsaturated molecules

In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W)(Q)(T)(Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05≤(b/c)≤100, and a≥0.

Chromatographic materials

The present invention provides methods for performing supercritical fluid chromatography comprising loading a sample to be separated by supercritical fluid chromatography onto a stationary phase comprising a spherical, monodisperse, core-shell particulate material comprising a nonporous core and one or more layers of a porous shell material surrounding the core, wherein the particles are sized less than 2 microns; and performing supercritical fluid chromatography to separate the sample.

Chromatographic columns and separation devices comprising a superficially porous material; and use thereof for supercritical fluid chromatography and other chromatography

Sorbents, devices, kits and methods useful for sample treatment are disclosed herein. In particular embodiments, described are inorganic/organic hybrid sorbent particles comprising (a) a core region that comprises a silica component and (b) a surface region that comprises an organic copolymer comprising at least one hydrophobic organic monomer and at least one hydrophilic organic monomer.

Sorbent particles for sample treatment

Described is a multi-stage pump having at least two stages. The multi-stage pump includes a first pump stage and a second pump stage each having a chamber of different diameter and volume. The multi-stage pump also includes a plunger having first and second plunger sections. The first plunger section has a first plunger diameter, a first end configured for coupling to a drive mechanism, and a second end opposite to the first end. The second plunger section has a second plunger diameter that is less than the first plunger diameter, a third end in contact with the second end of the first plunger, and a fourth end opposite the third end. Movement of the plunger produces a displacement volume for the first chamber that is different than a displacement volume produced for the second chamber. The multi-stage pump can be configured in different operational modes to provide different flow rates.

Multi-stage displacement pump

Techniques and apparatus for ion source devices with minimized post-column volumes are described. In one embodiment, for example, an ion source assembly may include a chromatography column in fluid communication with an ion source device, the chromatography column arranged within a minimum distance of the ion source, the minimum distance comprising between about 60 mm and about 150 mm.

Mass spectrometer ion source with integrated column

The present disclosure relates to the determination of pesticides, e.g., polar pesticides, in a sample using chromatography. The present disclosure can provide direct analysis of polar pesticides, including anionic polar pesticides, using high performance liquid chromatography. The polar pesticides are sufficiently retained and resolved to allow for multiple polar pesticide determinations in a single analysis.

Polar pesticide determination using chromatography

In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W)(Q)(T)(Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05&#x2264;(b/c)&#x2264;100, and a&#x2265;0.

The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group.

The present technology uses one or more separating segments, i.e. chromatography columns, aligned in series along a flow path. The separating segments are divided by a plurality of heating elements or are heated directly. The heating elements heat the supercritical mobile phase and sample to replace heat lost due to axial expansion of the mobile phase along the mobile phase flow path.

Separation efficiency in supercritical fluid chromatography

The subject technology is directed to a CO-based chromatography system and method for rapid determination of the levels and/or the presence or absence of steroids or steroid derivatives in a sample.

Growing community of inventors

Upton, MA, United States of America

Jacob Nathan Fairchild