Emulate, Inc.

Cedars-sinai Medical Center

Janssen Biotech, Inc.

Jacob Fraser

Daniel Levner

Christopher David Hinojosa

Geraldine A Hamilton

Norman Wen

S Jordan Kerns

Antonio Varone

Clive N Svendsen

Dhruv Sareen

Kyung Jin Jang

Dongeun Huh

Catherine Karalis

Riccardo Barrile

Justin Nguyen

Hyoungshin Park

David Conegliano

Andries Van Der Meer

Monicah Otieno

Carol Lucchesi

Gad Vatine

Robert N Barrett

Carolina Lucchesi

Magdalena Kasendra

Jefferson Puerta

Samuel Sances

Michael Workman

Stephen R Targan

Uthra Rajamani

Hyoung Shin Park

Chris Hinojosa

Ville Kujala

Samuel Sanees

SJordan Kerns

Berhan Mandefro

Anjoscha Kaus

Jordan Kerns

Magdelena Kasendra

The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.

Microfluidic model of the blood brain barrier

Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.

Systems and methods for growth of intestinal cells in microfluidic devices

Development of spinal cord on a microfluidic chip

Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed.

Compositions and methods of cell attachment

Compositions, devices and methods are described for preventing, reducing, controlling or delaying adhesion, adsorption, surface-mediated clot formation, or coagulation in a microfluidic device or chip. In one embodiment, blood (or other fluid with blood components) that contains anticoagulant is introduced into a microfluidic device comprising one or more additive channels containing one or more reagents that will re-activate the native coagulation cascade in the blood that makes contact with it 'on-chip' before moving into the experimental region of the chip.

Additive channels

Growing community of inventors

Somerville, MA, United States of America

Jacob Fraser