Gist-brocades B.v.

Gist-brocades N.v.

Unilever Patent Holdings B.v.

Danisco Ingredients A/s (danisco A/s)

Other

Dsm N.v.

Danisco US Inc.

Novartis Corporation

Danisco A/s

Ciba-geigy Corporation

Dsm IP Assets B.v.

Genencor International, Inc.

Dupont Nutrition Biosciences Aps

Dyadic Nederland B.v.

Jacob B Visser

Leendert Hendrik De Graaff

Henriette C Van Den Broeck

Johannes M Verbakel

Francois Strozyk

Felix J Kormelink

Johannes C Boonman

Wouter Musters

Abraham Harder

Hein Stam

Albert J Van Ooyen

Hermanus C Kester

Maria E Suykerbuyk

Johannes Heinrich Visser

Peter Jan Punt

Charlotte Horsmans Poulsen

Mark Aaron Emalfarb

Richard Paul Burlingame

Masoud Rajabi Zargahi

Albert J Van Ooijen

Peter Michael Andreoli

Jan Cornelis Verdoes

Christine Marie Pynnonen

Jan Wery

Albert J J Van Ooijen

Janna Gardina Bakhuis

Frank Buxton

Yvonne Muller

Phillip T Olson

Michel Johannes Flipphi

Marcus Matheus Gielkens

Monique Josina Van Der Wouw

Peter Van Der Veen

Yves Coutel

Gabor Jarai

Margaretha Van Heuvel

Henri&euml;tte C Van Den Broeck

Margo Anne-Rose Kusters-Van Someren

Ronald Peter De Vries

Claus Rolin

Henri&euml;tta Catharina Van Den Broeck

Petrus Jacobus Theodorus Dekker

Jutta Heim

Christof Gysler

Jan Maat

Bernd Meyhack

Birgit Michelsen

Albert Johannes Van Ooyen

Jean-Paul Meijnen

Paul William Schuurhuizen

Peter J Schaap

Peter J Van De Vonderwoort

Leendert Hendrick De Graaff

Albert Hinnen

Leendert H DeGraaff

Henrietta Catharina Van Den Broeck

Leendert H De Graaff

Martijn Johannes Koetsier

Sandra Wilhelmina Agnes Hinz

Mirjam Anna Kabel

Matthias Frommhagen

Harm Gruppen

Jurian Alexander Bronkhof

Carola Bianca Michielse

Ismail Atalay Van Der Burgt

Bouchra Benjelloun-Mlayah

Henriëtta Catharina Van Den Broeck

Danielle Basten

Petrus Johannes Schaap

Marcus Matheus Catharina Gielkens

No&euml;l Nicolaas Maria Elisabeth Van Peij

Jo Slashedrn So Slashede

Jan D Hille

Henriette Catharina Van Den Broeck

Jan Dirk René Hille

The present invention provides a new fungal production system comprising a fungal host strain ofwherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion ofstrain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous &#x3b2;-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion ofstrain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.

Fungal production system

The present invention is in the area of enzymes for (hemi-)cellulose degradation and/or modification, more in particular the degradation and/or modification of xylan. The invention is based on a newly discovered enzymatic activity of a class of lytic polysaccharide monooxygenases (LPMOs), i.e. oxidative cleavage of xylan in addition to oxidative cleavage of cellulose. The present invention therefore relates to a method for degrading and/or modifying xylan in a xylan-comprising substrate, a method for preparing a product from a xylan-comprising substrate, a kit of parts, a liquid, paste or solid formulation, and a xylan-comprising composition, comprising said LPMO. The invention further relates to a use of said LPMO, said kit of parts, said formulation and/or said composition, in a method of the invention.

Enzymatic activity of lytic polysaccharide monooxygenase

Provided are cells having an increased protein production characterized in that said cell comprises modified SUMOylation, a process for producing such a cell or expression system and the use of such a cell in producing a protein of interest.

Fungal high-level protein production system

The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. Knockout, mutants wherein a gene associated with metabolism is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.

Method to isolate mutants and to clone the complementing gene

The present invention describes isolated polypeptides from fungi which have aminopeptidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

Aspergillus phenylalanine aminopeptidase

The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. Knockout mutants wherein a gene associated with metabolism is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.

Recombinantly produced &agr;-glucuronidases which are useful in food manufacturing and as a feed additive to enhance the utilization of the feed components, and in other industrial applications such as pulp processing are provided. Genes coding for such enzymes can e.g. be isolated from Aspergillus sp. including or

&agr;-glucuronidases of aspergillus, production thereof and their uses

Two cellobiohydrolase polypeptides (CBHA and CBHB) derived from Aspergillus are described and can be used to degrade cellulose. Variants of these peptides are described as well as DNA encoding the peptides, vectors and host cells. The peptides can be used to produce or process food, animal feed, wood pulp, paper and textiles.

Cellulose degrading enzymes of aspergillus

The present invention provides polypeptides with cellulase activity, corresponding DNA sequences, vectors, transformed hosts and a method for the production of these polypeptides, which are obtainable from,or

Fungal cellulases

A nucleotide sequence is provided which encodes a peptide having &bgr;-xylosidase activity and exhibits at least 30% amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3 or hybridises under stringent conditions with a nucleotide sequence shown in SEQ ID NO. 1, or a part thereof having at least 15 nucleotides encoding an amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3. Also provided is a peptide having &bgr;-xylosidase activity and exhibiting at least 30% amino acid identity with the amino acid sequence shown in SEQ ID NO. 1 and described in SEQ ID NO. 3 or a part thereof having at least 8 amino acids shown in SEQ ID NO. 1 and described in SEQ ID NO. 3.

&bgr;-xylosidase, nucleotide sequence encoding it, and use thereof

The present invention relates to peptides with multiple enzymatic activitives: carboxymethyl cellusase(CMCase), endoglucanase and &bgr;-glucanase, corresponding nucleotides, vectors and transformed hosts, methods of preparation and use. The peptide is used in the food industry for the liquefaction of plant cell material, e.g., in vegetable or fruit juice manufacture, or as processing aids to reduce the fouling of membranes.

The subject invention lies in the field of microorganism mutation and selection of the mutants. In particular, the invention is directed at obtaining metabolic mutants in a simple, direct and specific manner. In a preferred embodiment it is also possible to obtain desired mutants not comprising recombinant DNA, thereby facilitating incorporation thereof in products for human consumption or application, due to shorter legislative procedures. The method according to the invention involves random mutation and specific selection of the desired metabolic mutant. A nucleic acid cassette comprising a nucleic acid sequence encoding a bidirectional marker, said nucleic acid cassette further comprising a basic transcriptional unit operatively linked to the nucleic acid sequence encoding the bidirectional marker and said nucleic acid cassette further comprising an inducible enhancer or activator sequence linked to the basic transcription unit in such a manner that upon induction of the enhancer or activator sequence the bidirectional marker encoding nucleic acid sequence is expressed, said inducible enhancer or activator sequence being driven from a gene associated with metabolism is claimed as is application thereof in a selection method for mutants. In addition a regular gene xlnR encoding an activating regulator of an inducible enhancer or activator sequence and application of said gene and/or its expression product in overexpression of homologous or heterologous protein or peptide is described. Knockout mutants wherein said gene is absent or inactivated and mutants with increased or decreased DNA binding capacity are also claimed.

An enzyme system is described that is useful for preparing food and feed. One enzyme of that system is obtainable from Aspergillus. That enzyme has the following characteristics: a MW of from 29 kDa to 36 kDa as measured on a SDS-Phastgel (10-15%) or about 30 kDa; a pI value of about 3-4; ferulic acid esterase activity; a pH optimum of about 5; and a temperature optimum of from about 50 to about 60.degree. C.

Enzyme system comprising ferulic acid esterase from Aspergillus

An arabinoxylan-degrading enzyme from Aspergillus is provided. The enzyme is active in the degradation of water-insoluble solids obtained from maize. The enzyme can be used in the preparation of animal feed compositions, human food or in industrial processes.

Arabinoxylan degrading enzymes

Growing community of inventors

Haastrecht, Netherlands

Jacob B Visser