Saint-gobain Performance Plastics Corporation

Jacob Andrews

ZhenWu Lin

Kenneth James Renfrew

Hannah C Hertrick

Phillippe Jean Broussard

Robert G Pleydon

Kenneth D Adrian

Natasha Anna Boghosian

Kenneth D Adnan

The disclosure relates generally to a cell culture apparatus and a cell culture method. One aspect of the disclosure provides a perfusion cell culture bag comprising one or more polymer films, having edges bonded together to form edges around an undivided interior compartment of the bag; first and second ports each formed in an exterior surface of the bag; first and second liquid-permeable tubes each extending into and in fluid communication with the interior compartment of the bag and operatively coupled to a respective port; and a third port formed in an exterior surface of the bag, the third port being in fluid communication with the interior compartment of the bag; wherein the first tube comprises a first inner support structure defining a central lumen of the tube and a first outer filter layer surrounding the first inner support structure.

Cell culture system

A liquid recovery filter assembly for recovering filtered liquid trapped within a core or downstream side of a filter element. Multiple embodiments each include a recovery port and a recovery filter in fluid communication with the core or downstream side of the filter element. The recovery port is opened following filtration operations to permit and to facilitate filtered liquid to flow from a downstream or outlet port, thus allowing recovery of liquid remaining in the filter core or downstream side following filtering operations. The recovery filter permits the introduction of pressurized gas to force the filtered liquids from the filter assembly without compromising the sterility and/or non-contaminant condition of the liquid. Additional aspects include exchangeable filter cartridges or filter elements in single and multi-round configurations, embodiments with aspiration tubes and dip tubes and still others with hydrophilic/hydrophobic recovery filters that function as filters and as valves for the recovery port.

Liquid recovery filter

A bio-reactive container and filter assembly to improve flow rates and mass transfer without increased pressure. An inlet filter membrane secured to the container defines a container chamber in combination with the container. An inlet filter membrane element may also be secured to the container to provide structural support to the membrane. An outlet membrane secured to the container defines a container chamber in combination with the container. An outlet filter membrane element may also be secured to the container to provide structural support to the outlet membrane. Inlet and/or outlet filters and/or filter elements are constructed to significantly increase surface area available for fluid and/or gas ingress and/or egress. Single and double layer membranes with mixtures of hydrophobic and hydrophilic characteristics are used to maximize perfusion and gasification/degasification of bio-reactive containers.

High gas flow rate bio-reactive container filter apparatus

A filter cartridge constructed to prevent an enclosed pleated filter from collapsing into the through-bores formed in a rigid core in high differential pressure applications. The core is modified with reduced-dimension bores to reduce the area of filter material not supported by the core. Alternatively, or in addition, a semi-rigid or rigid core wrap is superposed about the core to add further structural support to the pleated filter. The wrap is formed as a webbed structure with the thickness and spacing of the webbing segments adjusted to partially occlude the underlying core though-bores. This reduces the effective cross-sectional diameter of the through-bores to reduce the overall area of the pleated filter unsupported by the core. The through-bores may have modified shapes to maintain the area of the through-bore and reduce the distance of the through-bore center point from portions of the core wall defining the through-bore. The core also may be modified with clustered through-bores regions.

High pressure resistant filter

Disclosed is a tube/tube connector/capsule port assembly that eliminates a connection point in a filter capsule apparatus. A relatively soft tube is thermally or sonically bonded to a relatively hard tube connector that may have a tube receiving bore, frustoconical tube receiving channel or straight tube receiving channel. The tube connector is bonded to a filter capsule port. The manufacturing process can be either a one-step process bonding the tube, tube connector and capsule in one step, or a two-step process that binds the tube to the tube connector in the first step and then binds the tube/tube connector subassembly to a filter capsule housing during the housing's molding procedure. Single and dual-walled tubes may be used as well as single and dual-walled tubes having reinforcing material superposed about or embedded in the tube wall(s). A tube support collar is also disclosed.

Connection-free filter capsule apparatus

Disclosed is a disposable needle filter apparatus with a universal truncated syringe-receiving neck to enable the filtration of fluids/solutions aspirated into a needle-bearing syringe. The apparatus includes a needle seal stopper and needle stop to create a substantially airtight chamber to facilitate fluid aspiration through an enclosed filter and into the syringe. In an alternative embodiment, the filter apparatus includes an extended syringe-receiving neck configured to receive a specifically sized syringe/needle combination. In a further embodiment, the receiving neck is constructed as a modular unit to allow for interchangeable necks to accommodate different needle and syringe sizes with different cross-sectional diameters. In a yet further embodiment, the neck is constructed with stepped tapered segments of serially larger cross-sectional diameters to accommodate differently sized syringes. In a still further embodiment, the apparatus includes an accessory needle secured to the apparatus inlet.

Needle filter apparatus

The liquid recovery filter includes several embodiments, each of which provides for the recovery of liquid otherwise trapped within the filter housing and element. Each embodiment includes a series of vents, purge ports, and passages that communicate fluidly with the filter housing and filter core. Certain vents and purge ports are initially opened following filtration operations to allow pressurized gas to flow into the housing, thereby forcing residual unfiltered liquid through the filter. When this has been accomplished, another vent is opened to the filter core to allow filtered liquid to flow from the downstream or outlet passage from the filter core. Some embodiments have the filter outlet located above the filter, and include a downstream drain port extending from the filter core. The downstream drain port provides for the recovery of filtered liquid when the vent or purge port is opened in these embodiments.

Growing community of inventors

Washington, DC, United States of America

Jacob Andrews