Millipore Corporation

Codex Dna, Inc.

Jack Thacher Leonard

Joseph E Gabriels

Constance MacDonald

Markus Beier

Peer Friedrich Staehler

Joachim Jaeger

Raphael Carapito

Cord F Staehler

Mark Matzas

The invention relates to methods and devices for preparing synthetic nucleic acids.

Synthesis of sequence-verified nucleic acids

A process for the recovery of plasmids or other DNA from cells using a first filtration step to remove the cellular debris and other large cellular components and then an ultrafiltration step to capture the plasmids or other DNA on the surface of the ultrafiltration membrane where they may be recovered. An apparatus is also taught for enacting the process and comprises an upper microfiltration or coarse filtration membrane and a lower ultrafiltration membrane. The driving force may be the same for both filters or different and may be done sequentially or simultaneously.

Method of plasmid recovery and apparatus for doing so

A method for sequencing reaction cleanup adapted to remove contaminants from a sequencing reaction, comprising the steps of: providing a defined quantity of sequencing reaction product; providing at least one ultrafiltration membrane having at least one surface; transferring the suspended sequencing reaction product to the surface of the ultrafiltration membrane; and applying a first constant pressure differential to the ultrafiltration membrane at a force capable of producing the sequencing reaction product substantially free of the contaminants.

Method for sequencing reaction cleanup by constant differential pressure ultrafiltration

Method for sequencing reaction cleanup by constant pressure differential ultrafiltration

Method for sequencing reaction cleanup by constant pressure diffential ultrafiltration

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South Hamilton, MA, United States of America

Jack Thacher Leonard