Accuri Cytometers, Inc.

Jack T Ball

Collin Anderson Rich

Grant C Howes

Clare E Rogers

Aaron Kehrer

Nathaniel C Bair

A system and method for a flow cytometer system including a prepared sample fluid with reference beads; an interrogation zone that analyzes the prepared sample fluid; a peristaltic pump system that draws the sample fluid through the interrogation zone; and a processor that monitors a measured volume of sample fluid sampled by the peristaltic pump system and an expected sample volume based on data generated by the analysis of the sample fluid. A system and method is additionally described using an alternative volume sensing fluidic system.

System and method of verification of a prepared sample for a flow cytometer

A method for detecting fluorochromes in a flow cytometer, including: receiving a sample including particles each tagged with at least one of a first fluorochrome and a second fluorochrome, in which the first and second fluorochromes having distinct spillover coefficients; detecting the particles, including detecting the first and second fluorochromes with a first detector and a second detector; forming a data set for detected particles based on the detection of the first and second fluorochromes; characterizing a detected spillover coefficient for each detected fluorochrome from the data set; and sorting the detected particles into predicted fluorochrome populations based on the detected spillover coefficients. A system for detecting fluorochromes in a flow cytometer, including a flow cell, a fixed gain detection system, and a processor that generates a detected spillover coefficient for each detected particle and sorts the detected particle into predicted fluorochrome populations based on the detected spillover coefficient.

Method and system for detecting fluorochromes in a flow cytometer

The fluidic systemof the preferred embodiment includes a sheath pumpto pump sheath fluidfrom a sheath containerthrough a sample portinto an interrogation zoneand a waste pumpto pump the sheath fluidand a sample fluidas waste fluidfrom the interrogation zoneinto a waste container, and a processorto calculate a time window based on the flow rate of the sample fluid. Preferably the processoralso calculates a time window for the sample fluid to reach the interrogation zonefrom the sample portbased on the flow rate of the sample fluid. The interrogation zonefunctions to provide a location for the fluidic systemand an optical analysis systemof the flow cytometer to cooperatively facilitate the analysis of the sample fluid

Fluidic system for a flow cytometer with temporal processing

A method for cleaning a fluidic system of a flow cytometer having a sheath pump to pump sheath fluid towards an interrogation zone and a waste pump to pump the sheath fluid and a sample fluid as waste fluid from the interrogation zone, wherein the sheath pump and/or the waste pump draw sample fluid into the flow cytometer through a drawtube towards the interrogation zone. The method includes controlling the sheath pump and the waste pump to cooperatively flush a fluid out through the drawtube, thereby cleaning the fluidic system of the flow cytometer.

Fluidic system with washing capabilities for a flow cytometer

A system for a flow cytometer that collects data for a sample prepared with a plurality of fluorochromes that includes a fixed gain detection system that collects data for a plurality of fluorescence channels, fluorochrome compensation factors for a plurality of fluorochromes types, and a computer system that has an interface that gathers fluorochrome information of the sample and an analysis program that compensates for spectral spillover in the collected data. The fixed gain detection system preferably has a wide dynamic range. A fluorochrome compensation factor preferably remains constant for a fixed gain detection system. The analysis program preferably uses the fluorochrome compensation factors to compensate for spectral spillover.

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Ann Arbor, MI, United States of America

Jack T Ball