Codexis, Inc.

Pfizer Corporation

Tate & Lyle Solutions USA LLC

Bioavailability Systems, L.l.c.

Dsm Pharmaceuticals, Inc.

Jack S Liang

Emily Mundorff

Gjalt W Huisman

John M Gruber

Rama Voladri

Stephane J Jenne

James Joseph LaLonde

Jonathan Vroom

Anke Krebber

Charlene Ching

Nikki Dellas

David Entwistle

Jeffrey C Moore

Jovana Nazor

Stephan Jenne

Xiyun Zhang

Stephanie Sue Galanie

Vesna Mitchell

Ravi David Garcia

Joyce Liu

Matthew Truppo

Birgit Kosjek

Robert Osborne

Shane Grosser

Da Duan

Agustina Rodriguez-Granillo

Nicholas M Marshall

Robert Seemayer

Courtney Dianne Moffett

Iman Farasat

Jonathan S Penfield

Grant S Murphy

James LaLonde

John D Munger, Jr

Jian Ding

Scott J Novick

Benjamin Mijts

Lisa Marie Newman

Melissa Ann Mayo

Michael D Clay

Birthe Borup

Jagadeesh Mavinahalli

Roger Arthur Sheldon

Sheela Muley

Jeffrey Colbeck

Jun Zhu

Steven James Collier

Oscar Alvizo

Derek J Smith

Jacob M Janey

Fabien Louis Cabirol

Anupam Gohel

Gregory Hughes

Gregory Allan Cope

Artis Klapars

Gregory L Beutner

John Limanto

Luan Thanh Nguyen

Brendan T Grau

Eric Ashley

Thomas C Nugent

Mike Benoit

Marissa Mock

Alberto Ortega Guerra

Irene Fusman

Hallena R Strotman

Christopher Keltie Prier

Gjalt W Hiusman

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with β-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

Engineered glycosyltransferases and steviol glycoside glucosylation methods

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

Ketoreductase polypeptides for the reduction of acetophenones

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.

Ketoreductase polypeptides for the production of (R)-3-hydroxythiolane

The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

Biocatalysts and methods for hydroxylation of chemical compounds

Engineered proline hydroxylase biocatalysts for hydroxylation of chemical compounds

The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.

Ketoreductase polypeptides and polynucleotides

The present invention provides engineered glycosyltransferase (GT) enzymes, polypeptides having GT activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention provides engineered sucrose synthase (SuS) enzymes, polypeptides having SuS activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the GT enzymes and methods of using the engineered GT enzymes to make products with &#x3b2;-glucose linkages. The present invention further provides compositions and methods for the production of rebaudiosides (e.g., rebaudioside M, rebaudioside A, rebaudioside I, and rebaudioside D). The present invention also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

Vectors for expression of biocatalysts

Growing community of inventors

South San Francisco, CA, United States of America

Jack S Liang