New England Biolabs, Inc.

Jack S Benner, Ii

Phyllis A Rees

Shuang-Yong Xu

Zhenyu Zhu

Richard D Morgan

Theodore B Davis

Ellen P Guthrie

Toby E Claus

Linda H Coe

Recombinant DNA encoding NruI- and SboI-like restriction endonucleases and methylases and their amino acid sequences are provided as well as methods for expressing the enzymes in transformed host cells and purifying the enzymes.

Polynucleotides encoding polypeptides and host cells therefor

Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65&#xb0; C. and efficiency of dephosphorylation activity at approximately neutral pH.

Overexpression, purification and characterization of a thermolabile phosphatase

The present invention is directed to a method for cloning and producing the SspI restriction endonuclease by 1) introducing the restriction endonuclease gene from Sphaerotilus species into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the SspI restriction endonuclease, and 3) purifying the SspI restriction endonuclease from the fermented host which contains the vector encoding and expressing the SspI restriction endonuclease activity.

Method for producing the SSPI restriction endonuclease and methylase

The present invention is directed to a method for cloning and producing the NotI restriction endonuclease by 1) introducing the restriction endonuclease gene from Nocardia otitidis-caviarum into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity, and 3) purifying the NotI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NotI restriction endonuclease activity.

Isolated DNA encoding the NotI restriction endonuclease and related

The present invention is directed to a method for cloning and producing the Hpa I restriction endonuclease by 1) introducing the restriction endonuclease gene from Haemophilus parainfluenzae into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the Hpa I restriction endonuclease, and 3) purifying the Hpa I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Hpa I restriction endonuclease activity.

Method for producing the Hpa I restriction endonuclease and methylase

The present invention is directed to a method for cloning and producing the Nde I restriction endonuclease by 1) introducing the restriction endonuclease gene from Neisseria denitrificans into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the vector encoding and expressing the Nde I restriction endonuclease, and 3) purifying the Nde I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Nde I restriction endonuclease activity.

Method for producing the Nde I restriction endonuclease and methylase

The present invention is directed to a method for cloning and producing the Hinc II restriction endonuclease by (1) introducing the restriction endonuclease gene from Haemophilus influenzae Rc into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the Hinc II restriction endonuclease, and (3) purifying the Hinc II restriction endonuclease from the fermented host which contains the vector encoding and expressing the Hinc II restriction endonuclease activity.

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South Hamilton, MA, United States of America

Jack S Benner, Ii