Pioneer Hi-Bred International, Inc.

Monsanto Technology LLC

Other

Intrexon Corporation

Interexon Corporation

Jack Q Wilkinson

Karen L Fincher

Stanislaw Flasinski

Kevin E McBride

Sean M Bertain

Michael W Lassner

C Peter Romaine

Zhixin Shi

Donald S Walters

Stanilaw Flasinski

The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from thelectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

Regulatory element for heterologous protein production in the fruiting body of filamentous fungi

The present invention relates to novel hybrid promoters comprising a caulimovirus promoter operably linked to one or more of an EF1&#x3b1;, Act8, Act2 or Act11 promoter. The present invention also relates to novel DNA constructs comprising at least one expression cassette which comprises the hybrid promoter thereof. The present invention further relates to transgenic plants/seeds comprising such DNA constructs.

Hybrid caulimovirus promoters and constructs thereof

A stuffing box maintenance device designed to be releasably secured to a polished rod above the stuffing box wherein the stuffing box maintenance device is configured to retain the tools and the maintenance items required to perform a maintenance procedure on the stuffing box. The stuffing box maintenance device further includes a body being planar in manner and rectangular in shape. A mounting ring secured to the body on one end is operable to surroundably mount the polished rod. An opening is located on the mounting ring opposite the body and is configured to allow the stuffing box maintenance device to be laterally coupled to the polished rod. The stuffing box maintenance device further includes a support rod for retaining at least one packing gland. The body is further configured to retain the hand tools necessary to perform a maintenance procedure on the stuffing box.

Stuffing box maintenance device

The present invention provides recombinant expression cassettes comprising a fungal 3' termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and fungal termination sequence. The fungal 3&#x2032; termination sequence is heterologous to the coding sequence. The fungal 3&#x2032; termination sequence comprises structural features including a cleavage site, a positioning element. and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Recombinant expression cassettes with a fungal 3' termination sequence that function in plants

The present invention provides recombinant expression cassettes comprising a fungal 3' termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3&#x2032; termination sequence is heterologous to the coding sequence. The fungal 3&#x2032; termination sequence comprises structural features including a cleavage site, a positioning element and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

The present invention provides recombinant expression cassettes comprising a fungal 3' termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop codon, and the fungal termination sequence. The fungal 3&#x2032; termination sequence is heterologous to the coding sequence. The fungal 3&#x2032; termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

The present invention provides recombinant expression cassettes comprising a fungal 3' termination sequence which is functional in a plant. The recombinant expression cassettes comprise a plant promoter operably linked to a coding sequence having a stop condon, and the fungal termination sequence. The fungal 3&#x2032; termination sequence is heterologous to the coding sequence. The fungal 3&#x2032; termination sequence comprises structural features including a cleavage site, a positioning element, and an upstream element. The present invention also comprises methods for construction of the plant expression cassettes and introducing the cassettes into plant cells.

Recombinant expression cassettes with fungal 3' termination sequence that function in plants

The present invention provides novel plastid transit peptides that direct localization of attached moieties (e.g., polypeptides) into plant plastids. The present invention also relates to methods and compositions for localizing polypeptides to plant plastids including, but not limited to, transgenic plant production.

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Redwood City, CA, United States of America

Jack Q Wilkinson