Other

Research, Incorporated

Bethesda Research Laboratory

Jack G Chirikjian

Terence M Phillips

William F Feller

A method for diagnosing mammalian breast cancer by detecting in the physiological fluid of said mammal an antigen (ACRT) having immune cross-reactivity with human reverse transcriptase, or detecting antibodies against said ACRT or detecting antibody-ACRT complexes, wherein the reverse transcriptase is substantially purified, has a molecular of about 70,000 and a sedimentation coefficient on a glycerol gradient of between 5 and 5.5 S. A process for the purification of reverse transcriptase from human milk.

Reverse transcriptase from human milk, method for its purification, and

Restriction endonucleases are immobilized by chemically bonding the restriction endonucleases to water-insoluble matrices. The immobilized restriction endonucleases have enhanced stability and can be used to obtain deoxyribonucleic acid fragments in relatively low salt media, free of protein and ready for further use without tedious and time-consuming manipulations.

Immobilized restriction endonucleases

The disclosure is of fluorescent deoxyribonucleic acid intercalating agent derivatives of activated polysaccharides such as cyanogen halide-activated agarose and the like. The derivatives are useful fluorescent stain ingredients of water-insoluble gels used to fractionate deoxyribonucleic acids and deoxyribonucleic acid fragments by gel electrophoresis techniques. As ingredients of electrophoretic grade gels, the derivatives of the invention function as fluorescent stains to provide direct visualization of the deoxyribonucleic acids and their fractions, under the excitation of shortwave, ultraviolet radiation.

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Rockville, MD, United States of America

Jack G Chirikjian