Bloodcenter Research Foundation

Blood Center Research Foundation, Inc., the

Jack A Gorski

Lee Ann Baxter-Lowe

A method for HLA typing by amplification of a sample followed by sequence-specific oligonucleotide hybridization with a labelled oligonucleotide probe provides for both positive and negative controls. Control sequences representing known allelic polymorphisms at the locus in question are subjected to the labelled probe along with the sample. This method reduces errors and improves the chance of obtaining a successful tissue match, as is vital in the case of tissue transplants, particularly bone marrow transplants. Probes and PCR primers useful in HLA-DR typing are also provided.

Method for HLA typing

An approach to monitoring immune responses relies on determining the range of sizes of amplified DNAs which code for the CDR3 regions of Ig or TcR molecules of one or more classes or families. Typically the relative quantity of DNAs corresponding to different CDR3 regions of the Ig or TcR molecules of a class is also determined. The progress of an immune response is followed by making these determinations at different times.

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Mukwonago, WI, United States of America

Jack A Gorski