University of Georgia Research Foundation, Inc.

Jacek Gaertig

Theodore G Clark

Harry W Dickerson, Jr

Dorota Wloga

Donna Cassidy-Hanley

Swati M Suryavanshi

is used as a host cell in a biological assay for identification of modulators of tubulin ligases.

Biological system and assay for identifying modulators of tubulin ligases

The ciliated protozoanexemplifies a recombinant system for the expression of heterologous nucleic acids, preferably on the plasma membrane surface. Integration of a heterlogous nucleic acid into the &#x3b2;-tubulin gene, BTU1, of a paclitaxel-sensitivemutant that possesses btu1-IK350M &#x3b2;-tubulin allele allows screening for transformants using negative selection, as transformants have restored paclitaxel resistance. Transgenic ciliated protozoa of the invention can serve as live vaccines. For example, transgenicexpressingi-antigen protein on their surface are effective vehicles for vaccination of freshwater fish against infection by

Recombinant expression of heterologous nucleic acids in protozoa

The ciliated protozoan Tetrahymena exemplifies a recombinant system for the expression of heterologous nucleic acids, preferably on the plasma membrane surface. Integration of a heterlogous nucleic acid into the &#x3b2;-tubulin gene, BTU1, of a paclitaxel-sensitivemutant that possesses btu1-1K350M &#x3b2;-tubulin allele allows screening for transformants using negative selection, as transformants have restored paclitaxel resistance. Transgenic ciliated protozoa of the invention can serve as live vaccines. For example, transgenic Tetrahymena expressingi-antigen protein on their surface are effective vehicles for vaccination of freshwater fish against infection by

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Athens, GA, United States of America

Jacek Gaertig