Molecular Assemblies, Inc.

The Perkin-elmer Corporation

Other

Life Technologies Corporation

Applera Corporation

University of Ottawa

Fluidigm Corporation

Helicos Biosciences Corporation

Sequenom, Inc.

Efcavitch, J. William

J William Efcavitch

Steven Menchen

Stanley N Lapidus

Doron Lipson

Paul D Grossman

Ramakrishna S Madabhushi

Suhaib Siddiqi

Matthew T Holden

Kim F Albizati

Sanjay Agarwalla

Julie L Tubbs

Boguslaw Stec

Prem Sinha

Roger A O'Neill

Subramanian Marappan

Pascal Mayer

Claudia Chiesa

Jayson Linn Bowers

Robert Ruhfel

Natasha Paul

Judith Mitchell

Gary W Slater

Hong Yan Zhou

Guy Drouin

Jean Rousseau

Atanu Roy

Philip Richard Buzby

Edyta Krzymanska-Olejnik

Xiaopeng Bai

Christopher Wilson

John F Thompson

Alan Grubbs

Mirna Jarosz

Patrycja A Hopkins

Jay K Singh

Juliesta E Sylvester

Deanne W Sammond

Mirna Jaroza

Patrice M Milos

Stanley Letovsky

The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using renewable initiators coupled to a solid support. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template.

Reusable initiators for synthesizing nucleic acids

Nucleic acid memory strands encoding digital data using a sequence of homopolymer tracts of repeated nucleotides provides a cheaper and faster alternative to conventional digital DNA storage techniques. The use of homopolymer tracts allows for lower fidelity, high throughput sequencing techniques such as nanopore sequencing to read data encoded in the memory strands. Specialized synthesis techniques allow for synthesis of long memory strands capable of encoding large volumes of data despite the reduced data density afforded by homopolymer tracts as compared to conventional single nucleotide sequences.

Homopolymer encoded nucleic acid memory

The invention includes methods for identifying polymerases, such as modified terminal nucleotidyl transferases (TdT), that are capable of binding nucleotides comprising removable 3'-O-blocking moieties to a nucleic acid initiator, without the use of a template. The invention further includes the identified polymerases, and methods of using the polymerases for de novo synthesis of predetermined oligonucleotide sequences.

Modified template-independent enzymes for polydeoxynucleotide synthesis

The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using enzymes and specially designed nucleotide analogs. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template. Because the nucleotide analogs have an unmodified 3' OH, i.e., as found in 'natural' deoxyribose and ribose molecules, the analogs result in natural polynucleotides suitable for incorporation into biological systems.

Methods and apparatus for synthesizing nucleic acids

Methods and apparatus for synthesizing nucleic acid

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San Carlos, CA, United States of America

J William Efcavitch