J.r. Simplot Company

J Troy Weeks

Caius Rommens

Jingsong Ye

Hui Duan

Caius M Rommens

The invention relates to methods for stably integrating a desired polynucleotide into a plant genome, comprising transforming plant material with a first vector comprising nucleotide sequences encoding TAL proteins designed to recognize a target sequence; transforming the plant material with a second vector comprising (i) a marker gene that is not operably linked to a promoter ('promoter-free marker cassette') and which comprises a sequence homologous to the target sequence; and (ii) a desired polynucleotide; and identifying transformed plant material in which the desired polynucleotide is stably integrated.

TAL-mediated transfer DNA insertion

The present invention provides methods for producing transgenic plants based on an optimized transfer of DNA fromto plant cells, and/or on an optimized integration of the transferred DNAs into plant cell genomes. It also provides-transformation vectors that can be used to limit or eliminate the transfer of undesirable DNA. The present invention can be applied to essentially any species of plants, including many recalcitrant plant species.

Refined plant transformation

The present invention provides methods for enhancing plant transformation. One such method entails making an excision in a selected seedling at the point where two cotyledons meet, and then vortexing the cut seedling in a solution comprising a transforming bacterium such as

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Boise, ID, United States of America

J Troy Weeks