US Government As Represented by the Secretary of the Army

The State University of New York

J Robert Putnak

Kenneth H Eckels

Richard Lloyd Warren

Doria R Dubois

Charles H Hoke

Stephen J Thomas

Richard Jarman

Doris R Dubois

Kevin Cassidy

Timothy Endy

Rafael De La Barrera

Ashok Kumar Srivastava

The invention generally relates to a purified inactivated Zika virus (ZIKV), methods for producing the purified inactivated ZIKV, immunogenic compositions and vaccines comprising the purified inactivated ZIKV and methods for the prevention and/or treatment of infection by ZIKV.

Zika virus vaccine and methods of production

An inactivated dengue virus vaccine to immunize and protect humans against dengue fever is described. The vaccine is based on dengue viruses which have been propagated to high titers in suitable cells, purified and inactivated under conditions which destroy infectivity but preserve immunogenicity, a high level of which is demonstrated in animal models.

Inactivated dengue virus vaccine

An inactivated dengue virus vaccine to immunize and protect humans against dengue fever is described. The vaccine is based on dengue viruses which have been propagated to high titers in suitable cells, purified and inactivated under conditions which destroy infectivity but preserve immunogenicity, a high level of which is demonstrated in animal models. Uses of the inactivated dengue virus for detecting antibodies to dengue and kits therefor are also described.

Method and kit for detection of dengue virus

A recombinant protein encompassing a C-terminal protion from the structural envelope glycoprotein and an N-terminal portion from non-structural protein one of dengue type 2 virus was expressed in Escherichia coli as a fusion protein with Staphylococcal protein A. The recombinant protein was found to provide protection against lethal challenge with dengue 2 in mice.

Recombinant vaccine made in E. coli against dengue virus

Growing community of inventors

Silver Spring, MD, United States of America

J Robert Putnak