Visiongate, Inc.

University of Washington

Other

J Richard Rahn

Mark Fauver

Alan C Nelson

Eric J Seibel

Michael G Meyer

Thomas Neumann

Jon W Hayenga

Roger H Johnson

Michael J Meyer

Christy A Lancaster

Rahul Katdare

David Ethan Steinhauer

Anna V Tourovskaia

Julia Oi Yan Yu

John W Hayenga

Mark E Fauver

Michael Meyer

A method for 3D imaging of a biologic object () in an optical tomography system where a subcellular structure of a biological object () is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object () is moved relatively to a microscope objective () to present varying angles of view and the labeled biological object () is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object () and the microscope objective () within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object () from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object ().

Functional imaging of cells with optical projection tomography

A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

3D imaging of live cells with ultraviolet radiation

A system and method for detecting poor quality images in an optical tomography system includes an acquisition apparatus for acquiring a set of pseudo-projection images of an object having a center of mass, where each of the set of pseudo-projection images is acquired at a different angle of view. A reconstruction apparatus is coupled to receive the pseudo-projection images, for reconstruction of the pseudo-projection images into 3D reconstruction images. A quality apparatus is coupled to receive the 3D reconstruction images and operates to detect of selected features that characterize poor quality reconstructions.

System and method for detecting poor quality in 3D reconstructions

An optical tomography system for viewing an object of interest includes a microcapillary tube viewing area for positioning the object of interest in an optical path including a detector. A motor is located to attach to and rotate a microcapillary tube. A device is arranged for transmitting broadband light having wavelengths between 550 nm and 620 nm into the microcapillary tube viewing area. A hyperchromatic lens is located to receive light transmitted through the microcapillary tube viewing area. A tube lens is located to focus light rays transmitted through the hyperchromatic lens, such that light rays from multiple object planes in the microcapillary tube viewing area simultaneously focus on the at least one detector.

Depth of field extension for optical tomography

An optical tomography system for imaging an object of interest including a light source for illuminating the object of interest with a plurality of radiation beams. The object of interest is held within an object containing tube such that it is illuminated by the plurality of radiation beams to produce emerging radiation from the object containing tube, a detector array is located to receive the emerging radiation and produce imaging data used by a mechanism for tracking the object of interest.

Focal plane tracking for optical microtomography

A method for reconstructing three-dimensional (3D) tomographic images. A set of pseudo-projection images of an object is acquired. Error corrections are applied to the set of pseudo-projection images to produce a set of corrected pseudo-projection images. The set of corrected pseudo-projection images are processed to produce (3D) tomographic images.

Method for image processing and reconstruction of images for optical tomography

A scanning method for scanning samples of biological cells using optical tomography includes preparing, acquiring, reconstructing and viewing three-dimensional images of cell samples. Concentration and enrichment of the cell sample follows. The cell sample is stained. Cells are isolated from the cell sample and purified. A cell/solvent mixture is injected into a gel by centrifugation. A cell/gel mixture is injected into a capillary tube until a cell appears centered in a field of view using a stopped-flow method. An optical imaging system, such as a fixed or variable motion optical tomography system acquires a projection image. The sample is rotated about a tube axis to generate additional projections. Once image acquisition is completed, the acquired image projections are corrected for errors. A computer or other equivalent processor is used to compute filtered backprojection information for 3D reconstruction.

System and method for processing specimens and images for optical tomography

An optical projection tomography system is illuminated with a light source. An object-containing tube, a portion of which is located within the region illuminated by the light source, contains an object of interest that has a feature of interest. A detector is located to receive emerging radiation from the object of interest. A lens, including optical field extension elements, is located in the optical path between the object region and the detector, such that light rays from multiple object planes in the object-containing tube simultaneously focus on the detector. The object-containing tube moves relatively to the detector and the lens operate to provide multiple views of the object region for producing an image of the feature of interest at each view.

A system for optical imaging of a thick specimen that permits rapid acquisition of data necessary for tomographic reconstruction of the three-dimensional (3D) image. One method involves the scanning of the focal plane of an imaging system and integrating the range of focal planes onto a detector. The focal plane of an optical imaging system is scanned along the axis perpendicular to said plane through the thickness of a specimen during a single detector exposure. Secondly, methods for reducing light scatter when using illumination point sources are presented. Both approaches yield shadowgrams. This process is repeated from multiple perspectives, either in series using a single illumination/detection subsystem, or in parallel using several illumination/detection subsystems. A set of pseudo-projections is generated, which are input to a three dimensional tomographic image reconstruction algorithm.

Method and apparatus for pseudo-projection formation for optical tomography

Two or more two-dimensional Fourier transforms are acquired from different perspectives of a three-dimensional object region. A three-dimensional Fourier transform is then constructed using tomographic methods, permitting the application of image analysis algorithms analogous to those used for two-dimensional images.

Growing community of inventors

Sammamish, WA, United States of America

J Richard Rahn