Agilent Technologies, Inc.

Hewlett-packard Company

J Michael Flanagan

Steven Michael Fischer

Jian Bai

James L Bertsch

Darrell L Gourley

Joseph Vandenburg

Edward Aisawa

Val C Robinson

A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises: The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: 

Ambient pressure matrix-assisted laser desorption ionization (MALDI) apparatus and method of analysis

A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises: The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising:

A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises: The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.

Calibrant compositions are provided for use in either electrospray or APCI mass spectrometry, and can be used in either positive-ionization or negative-ionization modes. The compositions contain a primary calibrant preferably comprised of a mixture of homogeneously substituted triazatriphosphorines, and a secondary calibrant enabling calibration at relatively low mass-to-charge (m/z) values. The compositions enable calibration across a broad mass spectral range. Novel triazatriphosphorine compounds are provided as well, as are methods for calibrating mass spectrometers using the novel calibrant compositions.

Mass spectrometry calibration using homogeneously substituted

The invention relates to an ionization chamber. More particularly, the invention relates to a mass spectrometer system having an ionization chamber incorporating a corona needle removable from outside the closed ionization chamber. In preferred embodiments, the corona needle is self-positioning and engages and disengages operational or safety electrical connections.

Growing community of inventors

Sunnyvale, CA, United States of America

J Michael Flanagan