Becton Dickinson and Company Limited

Other

Beckon, Dickson and Company

J Bruce Pitner

James G Nadeau

C Preston Linn

Glenn P Vonk

Douglas Byron Sherman

Helen V Hsieh

Randal A Hoke

Ross Jacobson

James L Schram

G Terrance Walker

Jason E Gestwicki

Joseph Thomas

Terry J Amiss

Robert L Campbell

John Jacob Hemperly

Patrick Shawn Beaty

John D DeNuzzio

Kristin Weidemaier

Eric Fallows

Timothy G Foley, Jr

David T Stitt

Kevin G Dolan

Javier Alarcon

Richard David Guarino

Rajashaker Kache

Gregory J Burrell

Rosemary B Evans-Storms

Zachary A Pfieffer

Upma Gulati

Magdalena Wodnicka

Christopher C Herdman

Colleen M Nycz

David J Wright

Herman D Himel, Iv

Arounaguiry Ambroise

Larry M Gold

Patrick D Mize

Christopher J Knors

Steven Keith

Douglas P Malinowski

Cheryl H Dean

W Shannon Dillmore

Javier Alarcón

Terry J Amis

Linn, Jr C Preston

Pat D Mize

Herman Himel

Ross Wayne Jacobson

Some embodiments provided herein relate to combined assays. In some embodiments, an assay for identifying influenza type A or influenza type B is combined with an assay for determining the sensitivity of an influenza neuraminidase to an antiviral drug.

Methods and kits for the diagnosis of influenza

Method and kits for the diagnosis of influenza

The current invention relates to sensing elements and devices comprising at least one amphipathic lipid-binding protein or fatty acid binding protein, wherein the binding proteins are associated with a luminescent reporter group. The binding proteins and luminescent reporter groups are encapsulated within a hydrogel matrix that comprises at least one co-monomer, wherein the co-monomer is present at a concentration that decreases or inhibits micelle formation of the amphipathic lipid. Binding of the amphipathic lipid or fatty acid to the appropriate binding protein can produce at least one detectable change in the property of the luminescent reporter group.

Biosensor device for sensing amphipathic analytes

Reactive fluorescent dyes compositions and methods of using same are disclosed. Nile Red nucleus dyes are disclosed having thiol-reactive groups. Nile Red nucleus dyes are disclosed that exhibit a fluorescence emission of at least about 575 nm. The Nile Red nucleus dyes are of the following formulae: 

Long wavelength thiol-reactive fluorophores

Reactive fluorescent dyes compositions and methods of using same are disclosed. Squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed having thiol-reactive groups. Squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus dyes are disclosed that exhibit a fluorescence emission of at least about 575 nm. Biosensors are disclosed having a binding protein and a squaraine nucleus, Nile Red nucleus, benzodioxazole nucleus, coumarin nucleus or aza coumarin nucleus.

The invention is directed to compositions of mutated binding proteins containing reporter groups, analyte biosensor devices derived therefrom, and their use as analyte biosensors both in vitro and in vivo.

Binding proteins as biosensors

A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. An optical conduit, preferably an optical fiber has an optical system at the proximal end of the optical conduit. A sensing element is attached to the distal end of the optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the sensing element includes reference groups with luminescence properties that are substantially unchanged by variations in the analyte concentrations.

Fiber optic device for sensing analytes

A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is disclosed. A sensing element is attached to the distal end of an optical conduit, and comprises at least one binding protein adapted to bind with at least one target analyte. The sensing element further comprises at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the optical conduit and sensing element may be housed within a cannulated bevel.

Fiber optic device for sensing analytes and method of making same

The invention is directed to entrapped mutated binding proteins, mutated binding proteins containing reporter groups, compositions of mutated binding proteins containing reporter groups in analyte permeable matrixes, and their use as analyte biosensors both in vitro and in vivo.

Entrapped binding protein as biosensors

A device for sensing analyte concentration, and in particular glucose concentration, in vivo or in vitro is described. An optical conduit, preferably an optical fiber, has an optical system at the proximal end of the optical conduit. A sensing element is attached to the distal end of the optical conduit, and may include at least one binding protein adapted to bind with at least one target analyte. The sensing element further may also include at least one reporter group that undergoes a luminescence change with changing analyte concentrations. Optionally, the sensing element includes reference groups with luminescence properties that are substantially unchanged by variations in the analyte concentrations.

The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.

Luminescent method for determining cell oxygen consumption

The invention is directed to compositions of mutated binding proteins containing thiol groups for coupling to sensor surfaces, analyte biosensor devices derived there from, and methods of their use as analyte biosensors both in vitro and in vivo.

Growing community of inventors

Durham, NC, United States of America

J Bruce Pitner