Epigenomics Ag

Basf Se Corporation

Jürgen Distler

Kurt Berlin

Reimo Tetzner

Claude Taranta

Tatjana Sikuljak

Simon Nord

Fabian Model

Dimo Dietrich

Matthias Schuster

Alexander Olek

Ralf Lesche

Andrew Z Sledziewski

Thomas Bork

Richard A Warriner

Thomas Hildmann

Jörn Lewin

Xiaoling Song

Susan Cottrell

Tina Schröder-Grimonpont

Britta Katz

Michael Krouse

Shannon Payne

Daniel Bihlmeyer

David Gütig

Tamas Rujan

James Thomas Wofford

Theo Devos

Jesse Ho

Thomas L Skillman

Carolina Haefliger

Gunter Weiss

Andrew Morotti

Su Yamamura

Sharon Dekker

Volker Liebenberg

Yreka Ocampo

Rene Cortese

Andrew M Morotti

Kevin Day

Cathy Lofton-Day

Kevin J Day

Catherine Lofton-Day

Mathias Schuster

Jeffrey G Thomas

The invention relates to a method for analyzing cytosine methylations in DNA sequences, according to which non-methylated cytosines are first converted into uracil while 5-methylcytosine remains unmodified. The DNA is then amplified by means of a polymerase and at least one primer whose 5 end is connected to a probe via a linker. The probe is intramolecularly hybridized onto the amplified products in accordance with the methylation state of the DNA, hybridization being detectable via different detection systems. The inventive method is particularly suitable for diagnosing and predicting cancer diseases and other diseases associated with a modification of the methylation state as well as for predicting undesired effects of medicaments.

Method for the detection of cytosine methylations in DNA

A method is described for the amplification of nucleic acids, in which the segments to be amplified are first hybridized with at least two primer oligonucleotides that have two domains, wherein the sequence-specific domain found at the 3-end hybridizes to the segment to be amplified, while the generic domain found at the 5-end does not hybridize. Then an amplification reaction is conducted by means of a polymerase and subsequently a labeled primer oligonucleotide, which binds to the generic domain of the first primer, is hybridized to the amplificate which is formed. In the last step, the sequence of the amplificate is investigated.

Method for the simultaneous amplification of multiple sequences in a PCR reaction and marking thereof

The following invention concerns a method for investigating cytosine methylation by means of DNA repair enzymes. Here, the DNA is first converted so that unmethylated cytosines are converted to uracil, while 5-methylcytosine remains unchanged. Then the DNA is hybridized to oligonucleotides, whereby hybrids will be formed with or without erroneous base pairings, in each case depending on the methylation status of the DNA. Following this, the erroneously paired hybrids will be cleaved by repair enzymes. Then the methylation status of the DNA can be determined in different ways. The method according to the invention is particularly suitable for the diagnosis and prognosis of cancer disorders and other diseases associated with a change of the methylation status as well as for predicting undesired drug effects.

Method for investigating cytosine methylation in DNA by means of DNA repair enzymes

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Berlin, Germany

Jürgen Distler