Nch Corporation

Ivy Jones

Charles J Greenwald

John Knope

A system and method for generating an incubated bacteria solution by heating a nutrient germinant composition and bacteria, including at least one species in spore form, to a preferred temperature a range of 35-50&#xb0; C. for 2-60 minutes using exothermic chemical reaction heat. An incubated bacteria solution is preferably generated at or near a point-of-use in an aquaculture, agriculture, wastewater, or environmental remediation application. The nutrient-germinant composition comprises L-amino acids, optionally D-glucose and/or D-fructose, a buffer, an industrial preservative, and may include bacteria spores (preferably of one or morespecies) or they may be separately combined for incubation. A first chemical contained in a pouch is activated by contact with a second chemical, water, or air in a flameless heater to initiate exothermic reaction to provide incubation heat. A potable, single-use incubation bag is configured to hold the flameless heater and a container of nutrient germinant composition and spores.

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Coppell, TX, United States of America

Ivy Jones