Wisconsin Alumni Research Foundation

Bellbrook Labs, LLC

Invenra Inc.

Ivar Meyvantsson

David James Beebe

Michael W Toepke

Jay W Warrick

Glenn M Walker

Roland D Green

Bryan Glaser

John Majer

Kimberly Kaufman

Madison Green

Steven Hayes

Jay W Warwick

Methods for screening libraries of polypeptides for biologically activity on cells. For example, polypeptides can be synthesized and encapsulated along with their coding sequences in microcapsules of an emulsion. Emulsion microcapsules can then be fused with microcapsules comprising test cells and biological activity on the cells is assessed to identify biologically active polypeptides and nucleic acid molecules encoding the same.

High throughput screen for biologically active polypeptides

A method is provided for pumping fluid through a channel of a microfluidic device. The channel has an input port and an output port. The channel is filled with fluid and a pressure gradient is generated between the fluid at the input port and the fluid at the output port. As a result, fluid flows through the channel towards the output port.

Method of pumping fluid through a microfluidic device

A device and method is provided for performing a high throughput assay. The device includes a plate structure having a plate and a plurality of microfluidic structures positioned thereon. Each microfluidic structure defines a channel having an input and an output. At least one of the input and the output of the channel of each of the plurality of mircofluidic structures includes a first plurality of ports. In operation, the channels are filled with fluid and pressure gradients are generated between the fluids at the inputs and the fluids at the outputs of the channels. As a result, fluid flows through the channels toward the outputs.

Method for performing a high throughput assay

A microfluidic method and device for testing and analyzing chemotaxis by providing a stable, static fluid gradient. The device includes a sink reservoir for receiving biological cellular material and a source reservoir for receiving a chemoattractant. The biological cellular material migrates through a low fluid volume microfluidic gradient channel located between the source and sink reservoirs. The fluid in the gradient channel is static and stable due to a high fluid volume closed circuit bypass microfluidic channel also in fluid communication with the source and sink reservoirs, whereby the bypass channel relieves any pressure differential imparted across the gradient channel.

Microfluidic device having stable static gradient for analyzing chemotaxis

A method is provided of controlling communication between multiple ports in a microfluidic device. The method includes the step of providing a channel network in a microfluidic device. The channel network including a first channel having a first input port and an output port. The first channel is filled with a fluid and a first output droplet is deposited on the output port. The first output droplet has a radius of curvature. The first output droplet flows toward the first input port in response to placement of a first input droplet having a radius of curvature greater than the radius of curvature of the first output droplet on the first input port. The first input droplet flows toward the output port in response to the first input droplet having a radius of curvature less than the radius of curvature of first output droplet.

Method for controlling communication between multiple access ports in a microfluidic device

A method of quantifying cell migration of a cell population is provided. The method includes the step of patterning the cell population within a channel network in a first body. A first image of the cell population is obtained. Thereafter, a second image of the cell population is obtained after a first predetermined time period. The first and second images are compared in order to calculate a quantitative measure of the average directional migration of the cells population and a quantitative measure of the average motility of the cell population.

Growing community of inventors

Madison, WI, United States of America

Ivar Meyvantsson