Johns Hopkins University

Albert Einstein College of Medicine

Ivana Celic

Vern L Schramm

Jef D Boeke

Anthony Sauvé

Terry Harrison Shelley

Alex Leo Kolodkin

Shih-Hsiu Wang

A floating chamber set may include a plurality of containers coupled to a frame such that each container translates vertically within a limited vertical range independent of the other containers. Each container may be perforated to allow liquid penetration.

Sample processing device

A novel compound, 2'/3&#x2032;-O-acetyl-ADP-ribose, is provided. The compound is a mixture of the 2&#x2032; and 3&#x2032; regioisomers of O-acetyl-ADP ribose, and is formed nonenzymatically from 2&#x2032;-O-acetyl-ADP-ribose, which is the newly discovered product of the reaction of Sir2 enzymes with acetylated peptides and NAD. Analogs of 2&#x2032;/3&#x2032;-O-acetyl-ADP-ribose are also provided. Additionally, methods of preparing 2&#x2032;/3&#x2032;-O-acetyl-ADP-ribose, methods of determining whether a test compound is an inhibitor of a Sir2 enzyme, methods of detecting Sir2 activity in a composition, methods of deacetylating an acetylated peptide, and methods of inhibiting the deacetylation of an acetylated peptide are provided. Prodrugs of 2&#x2032;/3&#x2032;-O-acetyl-ADP-ribose are also provided.

Sir2 products and activities

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Baltimore, MD, United States of America

Ivana Celic