Contipro A.s.

Contipro Biotech S.r.o.

Ivana Basarabova

Vladimir Velebny

Radovan Buffa

Petra Sedova

Kristina Nesporova

Hana Vagnerova

Jaromir Kulhanek

Josef Chmelar

Katerina Knotkova

Helena Krejci

Lubos Sobotka

Lucie Wolfova

Tomas Bobula

Pavlina Prochazkova

Jaroslav Novotny

Drahomira Chladkova

Martina Hermannova

Robert Pospisil

Iva Doleckova

Martina Hasova

Sona Moravcikova

Vit Svozil

Veronika Stepankova

Lucie Marholdova

Tereza Ehlova

Michaela Moravkova

Ondrej Kotland

Kristyna Lipenska

Leos Hromek

Stanislav Pepeliaev

Tomás Pitucha

Martin Sojka

Tomas Pitucha

Martina Moravcova

Tereza Silova

A chlorinated derivative of hyaluronic acid or of a modified hyaluronic acid (chloramide) is provided. The chloramide has an amidic group (&#x2014;NH&#x2014;CO&#x2014;). The hydrogen of the amidic group is substituted by a chlorine atom, according to the structural formula &#x2014;NCl&#x2014;CO&#x2014;. The substitution degree of the hyaluronic acid or of the modified hyaluronic acid by chlorine is in an amount of from 50 to 100 %.

Chlorinated derivative of hyaluronic acid, method of preparation thereof, a composition containing the derivative, and use thereof

An antimicrobial composition is provided. The composition comprises a chloramid of hyaluronic acid or of modified hyaluronic acid. The chloramid has an amidic group (&#x2014;NH&#x2014;CO&#x2014;). The hydrogens of the amidic group are substituted by chlorine atoms according to the structural formula &#x2014;NCl&#x2014;CO&#x2014;. The composition further comprises an iodide. The substitution degree of the hyaluronic acid or of the modified hyaluronic acid by chlorine is in an amount of from 50% to 100%.

Composition comprising an iodide and a derivative of hyaluronic acid with an oxidative effect, method of preparation thereof and use thereof

The invention relates to the preparation of new polysaccharide derivatives comprising a double bond in the positions 4 and 5 of the pyranose cycle. The method of preparation consists in the oxidation of OH group in the position 6 to an aldehyde, followed by the elimination to form a double &#x2014;C&#x2550;C&#x2014; bond in the positions 4 and 5, and the final reduction of the aldehyde group in the position 6 into the original alcohol. The derivatives of polysaccharides prepared according to the invention show an enhanced antioxidant activity and some of them also a selective negative influence on carcinoma cell viability. (formula) where R represents &#x2014;NH&#x2014;CO&#x2014;CHor &#x2014;OH. 

Unsaturated derivatives of polysaccharides, method of preparation thereof and use thereof

The invention relates to conjugates of hyaluronic acid oligomer according to the general formulae I, II III or IV, or a salt thereof, the method of preparation thereof and use thereof, where the oligomer is bonded to the respective substrate by its ending anomeric center via a bi-functional amino linker by means of an amino or imino bond. This type of conjugates allows releasing oligomers in their native form. The prepared systems exhibited an enhanced biological activity against selected lines of cancer cells. 

Conjugates of oligomer of hyaluronic acid or of a salt thereof, method of preparation thereof and use thereof

The invention relates to the preparation and use of &#x3b1;,&#x3b2;-unsaturated aldehyde of hyaluronan having a double bond in the positions 4 and 5 and an aldehydic group in the position 6 of the glucosamine part of the polysaccharide. The method of preparation is based on dehydration of hyaluronan having an aldehydic group in the position 6 of the glucosamine part of the polysaccharide. Two methods have been described, which are dehydration in a solution or heating in solid state in absence of solvents, bases or other additives. This derivative allows stabilization of conjugates of hyaluronan with amino compounds by means of a multiple bond from the aldehyde side, and therefore, it is possible to effectively immobilize practically any compound containing an amino group to such modified hyaluronan in physiological conditions. In case of using a diamine or compounds or polymers containing three or more amino groups, it is possible to prepare crosslinked hyaluronan derivatives. The described solution brings along a significant advantage not only in the field of carriers of biologically active substances, but also in tissue engineering where crosslinking with biologically acceptable amino compounds in physiological conditions is very much demanded. 

Hyaluronic acid derivative, method of preparation thereof, method of modification thereof and use thereof

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Medzilaborce, Slovakia

Ivana Basarabova