Novo Nordisk A/s

Novozymes A/s

Nna/s

Ivan Verner Diers

Per Balschmidt

Jan Markussen

Michi Egel-Mitani

Torben Halkier

Ib Jonassen

Lisbeth Hedegaard

Thomas Borglum Kjeldsen

Pau Ferrer

Thomas Hoeg-Jensen

Erling Guddal

Asser Sloth Andersen

Juan A Asenjo

Henning Tho Slashedgersen

Anne Grete Byskov

Lars Nordholm

Claus Yding Andersen

Ole Wassmann

Demitris Savva

Henning Thphigersen

The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine &#x3b5;-amino groups.

Method for making acylated polypeptides

A process for producing high amount of proteins or polypeptides in yeast is disclosed. The process makes use of the CIT1 yeast promoter or a functional part or variant thereof. Examples of polypeptides which are expressed in high yields are insulin or insulin analogues or GLP1.

Production of heterologous polypeptides in yeast

The present invention relates to a method for extractive refolding of scrambled and/or polymerised single-chain polypeptides or proteins into their native conformation directly from a microbial fermentation broth.

Method for extractive refolding of scrambled single-chain polypeptides

The present invention relates to a DNA construct exhibiting &bgr;-1,3-glucanase activity, which DNA sequence comprises: a) the DNA sequence shown in SEQ ID No. 1 or SEQ ID No. 12, or b) an analogue of the DNA sequence defined in a), which i) is homologous with the DNA sequence defined in a), or ii) hybridizes with the same oligonucleotide probe as the DNA sequence defined in a), or iii) encodes a polypeptide which is homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence defined in a), or iv) encodes a polypeptide which is immunologically reactive with an antibody raised against the purified &bgr;-1,3-glucanase derived from,LLG109 encoded by the DNA sequence defined in a). The DNA construct may further encode a mannose binding domain. The invention also relates to a DNA construct encoding a mannose binding domain. Further the invention relates to an expression vector comprising said DNA constructs, a cell harbouring said DNA constructs or expression vectors, a method of producing the novel enzyme, a novel enzyme preparation containing said novel enzyme, the use of the enzyme for degrading or modification of &bgr;-glucan containing material.

Enzyme with &bgr;-1,3-glucanase activity

A DNA construct exhibiting .beta.-1,3-glucanase activity, including an expression vectors, cells harbouring the DNA construct or expression vector, a method of producing the enzyme, as well as the enzyme and preparations containing the enzyme.

Enzyme with B-1, 3-glucanase activity

The present invention relates to certain sterol derivatives for use as medicaments.

Regulation of meiosis using sterols

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Ivan Verner Diers