Braskem S.a.

Other

Genomatica, Inc.

Iuri Estrada Gouvea

Lucas Pedersen Parizzi

Veronica Leite Queiroz

Paulo Moises Raduan Alexandrino

Avram Michael Slovic

Mateus Schreiner Garcez Lopes

Daniel Johannes Koch

Felipe Cicaroni Fernandes

Debora Noma Okamoto

Felipe Galzerani

Verônica Leite Queiroz

Rafael Victório Carvalho Guido

Alessandro Silva Nascimento

Igor Polikarpov

Bárbara Mano

Marcos Rogerio Simoes

Aline Silva Romão Dumaresq

Johana Rincones Perez

Robert Haselbeck

Harish Nagarajan

Mateus Schreiner Lopes

Stephanie J Culler

Ana Karina Brambilla Costa

Marcos Rogerio Simões

Mariana Trovo Marchesin

Antonio Rodolfo, Jr

Rafael Victorio Carvalho Guido

Mateus Schreiner Garcez Lopez

Johanna Rincones Perez

The present disclosure provides recombinant microorganisms and methods for the production of 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA from a carbon source. The method provides for engineered microorganisms that express endogenous and/or exogenous nucleic acid molecules that catalyze the conversion of a carbon source into 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA. The disclosure further provides methods of producing polymers derived from 4-HMF, 2,4-furandimethanol, furan-2,4-dicarbaldehyde, 4-(hydroxymethyl)furoic acid, 2-formylfuran-4-carboxylate, 4-formylfuran-2-carboxylate, and/or 2,4-FDCA.

Method for the in vivo synthesis of 4-hydroxymethylfurfural and derivatives thereof

The present disclosure generally relates to a compound of dialkyl ester of 2,4-furandicarboxylic acid, a method of preparing the compound, a polymer composition comprising a polymer and the compound, a method of preparing the polymer composition, a polymer product comprising the polymer composition and a method of using the compound as a plasticizer in the polymer product. The dialkyl ester of 2,4-furandicarboxylic acid of the present disclosure has greater plasticizing efficiency in a polymer composition that that of the standard phthalate and terephthalate-based plasticizers. The polymer product plasticized with the dialkyl ester of 2,4-furandicarboxylic acid may have improved flexibility, durability, processability and safety as compared to the same polymer product plasticized with conventional phthalate and terephthalate-based plasticizers.

Production and use of plasticizers based on 2,4-isomer of furandicarboxylic acid diesters

The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

NADH-dependent enzyme mutants to convert acetone into isopropanol

The present disclosure provides a copolyester including a dicarboxylic acid component A comprising a terephthalic acid residue or ester-forming derivative thereof, or a mixture thereof, and a 2,4-furandicarboxylic acid residue or ester-forming derivative thereof, or a mixture thereof, and a diol component B comprising an alkanediol residue having from 2 to 22 carbon atoms, wherein the dicarboxylic acid component A has a total molar content, and wherein the 2,4-furandicarboxylic acid residue or ester-forming derivative thereof, is present in an amount of from 0.1 to 10 mol %, with respect to the total molar content of the dicarboxylic acid component A. Inventive copolyesters have a slower crystallization rate, a higher gas barrier to COand Oand a higher ratio ofC toC as measured by ASTM D6866 when compared to a comparable copolyester comprising isophthalic acid instead of 2,4-furandicarboxylic acid.

Copolyesters of 2,4-FDCA with increased crystallization control

The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandicarboxylic acid from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandicarboxylic acid with ethanol and for co-producing 2,4-furandicarboxylic acid with ethanol and 1-propanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandicarboxylic acid and that coupled the 2,4-furandicarboxylic acid pathway with an additional metabolic pathway.

Anaerobic fermentative production of furandicarboxylic acid

The present disclosure provides recombinant microorganisms and methods for the anaerobic production of 2,4-furandimethanol from one or more carbon sources. The microorganisms and methods provide redox-balanced and ATP positive pathways for co-producing 2,4-furandimethanol with ethanol and for co-producing 2,4-furandimethanol with ethanol and acetone and/or isopropanol. The method provides recombinant microorganisms that express endogenous and/or exogenous nucleic acid molecules encoding polypeptides that catalyze the conversion of a carbon source into 2,4-furandimethanol and that couple the 2,4-furandimethanol pathway with an additional metabolic pathway. The present disclosure further provides enzymatic production of 2,4-furandicarboxylic acid.

Anaerobic fermentative production of furandimethanol and enzymatic production of furandicarboxylic acid

The present application relates to recombinant microorganisms expressing a dehydratase useful in a one-step, direct fermentative production of one or more primary alkenes from one or more saturated primary or secondary alcohols. Known, well developed high-yielding pathways that use renewable feedstock can be introduced into the recombinant microorganisms to obtain the alcohol precursors. Also provided are methods of producing one or more primary alkenes using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the primary alkene products.

Formation of alkenes through enzymatic dehydration of alkanols

In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

Vinylisomerase-dehydratases, alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases and methods for making and using them

The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10&#xd7; higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.

Engineered enzyme having acetoacetyl-CoA hydrolase activity, microorganisms comprising same, and methods of using same

The present disclosure generally relates to methods of using microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene and products and processes derived therefrom.

Modified microorganisms and methods of making butadiene using same

Growing community of inventors

Campinas, Brazil

Iuri Estrada Gouvea