Asahi Kasei Pharma Corporation

Ichibiki Co., Ltd.

Issei Yoshioka

Takuji Kouzuma

Shigeyuki Imamura

Junichi Sumitani

Motoo Arai

Takeshi Matsuoka

Toshihiko Kumazawa

Atsuhisa Nishimura

Shinji Koga

Homare Itou

Takashi Kuroyanagi

Homare Ituo

Composition for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) siabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.

Composition for assaying glycated proteins

There is provided a method for specifically determining a glycated &#x3b2;-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated &#x3b2;-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated &#x3b1;-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated &#x3b2;-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated &#x3b2;-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.

Hemoglobin A1c determination method, enzyme to be used therefor, and production method thereof

Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) siabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.

Composition for assaying glycoprotein

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Shizuoka, Japan

Issei Yoshioka