Japan Atomic Energy Institute

Issay Narumi

Katsuya Satoh

Seiichi Wada

Tomoo Funayama

Yasuhiko Kobayashi

Masahiro Kikuchi

Zhenli Tu

The present invention is directed to directly measure distribution in vivo and the frequency of generation in vivo of DNA strand breaks which induce cell death and mutations. The present inventors accomplished the present invention by providing a method for detecting a DNA strand break in a sample, which comprises a step of binding a PprA protein derived fromto a DNA strand break and a step of detecting the PprA protein which is bound to the DNA strand break; as well as by providing a kit for detecting a DNA strand break in a sample which comprises PprA proteins derived fromand a means for detecting a PprA protein which is bound to a DNA strand break.

Method for efficiently determining a DNA strand break

The endogenous plasmid pUE30 from radioresistant bacterium strain ATCC19172 or a derivative thereof is used as a vector that can autonomously replicate in bacteria of the genus Deinococcus. It may be used to construct a shuttle vector that also contains a plasmid capable of autonomous replication in or a derivative thereof and which can replicate in both a bacterium of the genus Deinococcus and

Radiation resistant bacterium/E. coli shuttle vector

Growing community of inventors

Gunma, Japan

Issay Narumi