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Centre National De La Recherche Scientifique

Institut Curie

Université Pierre Et Marie Curie

Bockelmann, Ulrich

Ismaïl Cisse

Ulrich Bockelmann

Jean-Louis Viovy

Laurent Malaquin

Stephanie Descroix

Bastien Venzac

Virgile Viasnoff

François Amblard

Mohamed Lemine Diakite

The present invention concerns a method of detecting macroions in a liquid medium contained in a space, the method including: a) submitting the liquid medium to a stimulating electrical field to induce formation of aggregates of macroions, the formed aggregates of macroions preferably not including any additional labeling agent, and b) measuring, in a detection zone of the space, spatial and/or temporal fluctuations within the liquid medium of at least one variable depending on the concentration of the macroions in the liquid medium, and c) determining, based on these fluctuations, the presence of the macroions, step c) preferably including processing by a time-dependent or space dependent analysis, more preferably by wavelet analysis, or by autocorrelation the fluctuations measured at step b).

Method and devices for detecting macroions in a liquid medium

The present invention relates to a method for detecting a circularized single-stranded DNA by means of the isothermal hyperbranched rolling circle amplification technique, in which the primers used comprise a detectable barcode sequence and, optionally, a spacer which blocks polymerization by DNA polymerase. The present invention also relates to the use of said method for detecting a genetic polymorphism of one or more base pair(s).

Method for detecting a circularized DNA, and use of said method for detecting mutations

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Aubervillers, France

Ismaïl Cisse