Fujirebio Inc.

Fuji Photo Film Company, Limited

Other

Fujirebio Kabushiki Kaisha

Fujirejiro Inc.

Isao Nishizono

Yoshihiro Ashihara

Masahisa Okada

Yasusuke Sakurabayashi

Hidetaka Minakawa

Shin-ichi Wakana

Fumio Watanabe

Yukio Sudo

Tetsuji Tanimoto

Shigeki Kageyama

Toshikage Hiraoka

Yoshihiro Kurano

Kaoru Nakatsubo

Sachiko Kitajima

An immunoassay for selectively measuring human C-peptide as well as a kit therefor is disclosed. In the method, human C-peptide contained in a sample, a first anti-human C-peptide antibody, and a second anti-human C-peptide antibody which is immobilized on a solid support are reacted to form an immune complex among these three components. The formed immune complex is separated from the non-reacted antibodies and sample; and then the separated immune complex is quantified. The first antibody recognizes an epitope existing in the region from 1st to 16th amino acid residue from the N-terminal of the human C-peptide, and the second antibody recognizes an epitope existing in the region from 1st to 16th amino acid residue from the N-terminal of human C-peptide; with the proviso that the first and second antibodies do not recognize the same epitope so that they can simultaneously bind to said human C-peptide.

Immunoassay for measuring human C-peptide and kit therefor

A dry-type analytical element for immunoassay having at least one water-permeable layer for measuring a ligand in a sample according to enzyme immunoassay, which comprises,

Dry-type analytical element for immunoassay

An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well of said wells containing solid phase material carrying antigen or antibody, a second well of said wells containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film before use and the sealing film is broken when the cartridge is to be used. The cartridges are transported to a predetermined position on a steppingly movable reaction line and conveyed thereby at a predetermined interval. While the reaction line steps, a sample, labelled antigen or antibody contained in the second well and substrate, if necessary, are added to the first well and stirred at predetermined timings and reaction between the sample and a reactive solution is measured under control of a control device having memory means storing operator selectable programs for various measuring methods.

Automatic immunological measuring system

An automatic immunoassay apparatus utilizes cartridges each having at least two wells, a first well containing solid phase material carrying antigen or antibody, and a second well containing antibody or antigen labelled with labelling compound. The wells may be sealed with a suitable sealing film before use and the sealing film is broken when the cartridge is to be used. The cartridges are transported to a predetermined position on a steppingly movable reaction line and conveyed thereby at a predetermined interval. While the reaction line steps, a sample, labelled antigen or antibody contained in the second well and substrate, if necessary, are added to the first well and stirred at predetermined timings, and reactions between the sample and a reactive solution are measured under control of a control device having a memory storing operator selectable programs for various measuring methods.

Method of immunoassay measurement

Growing community of inventors

Tokyo, Japan

Isao Nishizono