Chugai Seiyaku Kabushiki Kaisha

Isamu Terashima

Katsuhiro Kano

Hiroyuki Doi

Seiko Yamamoto

The present invention provides a method of cell culture comprising adding a cell-containing seed medium to an initial medium and starting to culture the cell in the initial medium, wherein the initial medium has an organism-derived culture medium additive added thereto and the amount of the C-terminal amidated species in the produced protein is controlled by the copper content of the initial medium at the start of cell culture.

Process of production with controlled copper ions

An antibody subtype (1) which is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which one C-terminal of the heavy chain is Pro-NH(447), and an antibody subtype (2) which is a subtype of the humanized PM-1 antibody against interleukin-6 receptor (IL-6R) and in which both C-terminals of the heavy chain are Pro-NH(447), and a pharmaceutical composition comprising them.

Methods for producing subtypes of humanized antibody against interleukin-6 receptor

Subtypes of humanized antibody against interleukin-6 receptor

As a subtype of humanized PM-1 antibody against interleukin-6 receptor (IL-6R), there is provided antibody sub-type (1) whose one heavy chain C terminus consists of Pro-NH(447). As another subtype of humanized PM-1 antibody against interleukin-6 receptor (IL-6R), there is provided antibody subtype (2) whose both heavy chain C termini consist of Pro-NH(447). Furthermore, there are provided pharmaceutical compositions comprising these.

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Tokyo, Japan

Isamu Terashima