Metabolic Explorer

Centre National De La Recherche Scientifique

Institut National De La Recherche Agronomique

Institut National Des Sciences Appliquees

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Philippe Soucaille

Benjamin Gonzalez

Isabelle Meynial-salles

Isabelle Meynial-Salles

Rainer Figge

Michel Chateau

Laurence Dumon-Seignovert

Christian Croux

François Voelker

Olivier Zink

Céline Foulquier

Antoine Riviere

Ngoc-Phuong-Thao Nguyen

Philippe Noel Paul Soucaille

Benjamin Percheron

Francois Voelker

Philippe Nöel Paul Soucaille

Celine Foulquier

The present invention relates to a novel polypeptide having the enzymatic activity of reduction of NADP+ using electrons from reduced ferredoxin (Ferredoxin-NADP+ reductase activity), a polynucleotide having a nucleotide sequence encoding such polypeptide and uses thereof. The invention relates to the modulation of the Ferredoxin NADP+ reductase activity in a microorganism by varying the expression level of the polynucleotide coding for such polypeptide. The invention also relates to the production of commodity chemicals, especially ethanol, n-butanol, 1,3-propanediol, 1,2-propanediol, isopropanol and acetone by fermenting microorganisms wherein their Ferredoxin NADP+ reductase activity is modulated.

Polypeptide having ferredoxin-NADP+ reductase activity, polynucleotide encoding the same and uses thereof

The present invention relates to a new strain ofmodified to be unable to produce hydrogen and its use for the continuous production of bulk chemicals such as lactate, 1,3-propanediol, ethanol, butanol, isobutanol, 1,3-butanediol, acetate, acetone, isopropanol, 3-hydroxy-3-methylbutyrate and isobutene at high yield.

strains unable to produce hydrogen and useful for the continuous production of chemicals and fuels

The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a microorganism modified for an improved production of 1,2-propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced. The present invention also relates to a mutant glycerol dehydrogenase (GlyDH) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein

Mutant glycerol dehydrogenase (GlyDH) for the production of a biochemical by fermentation

The present invention concerns a new method of preparation of a strain of evolved micro-organisms for the production of 1,2-propanediol by the metabolism of a simple carbon source, which method comprises the growth under selection pressure in an appropriate growth medium containing a simple carbon source of an initial bacterial strain that has undergone the deletion of the gene tpiA and the deletion of at least one gene involved in the conversion of methylglyoxal (propanal) into lactate, in order to cause, in said initial strain, the evolution of one or more genes involved in the biosynthesis pathway from DHAP to methylglyoxal and then to 1,2-propanediol towards evolved genes that possess an improved '1,2-propanediol synthase activity', the resulting strain or strains of evolved micro-organisms possessing an improved '1,2-propanediol synthase activity' then being selected and isolated. The invention also concerns the initial micro-organisms and the evolved micro-organisms thus obtained, and a method for the preparation of 1,2-propanediol and possibly acetone by culture of said evolved micro-organisms.

Evolved micro-organisms for the production of 1,2-propanediol

The present invention is directed to a method for the preparation of evolved microorganisms permitting the creation or modification of metabolic pathways. Strains of evolved microorganisms and evolved genes obtained by the method are also provided. The invention is also directed to the use of evolved microorganisms, genes or proteins in a biotransformation process.

Method for the preparation of an evolved microorganism for the creation or the modification of metabolic pathways

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Fourquevaux, France

Isabelle Meynial-Salles